OCRL Antibody - #DF8585
| 製品: | OCRL Antibody |
| カタログ: | DF8585 |
| タンパク質の説明: | Rabbit polyclonal antibody to OCRL |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 104 kDa; 104kD(Calculated). |
| ユニプロット: | Q01968 |
| RRID: | AB_2841789 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8585, RRID:AB_2841789.
折りたたみ／展開
EC 3.1.3.36; Inositol polyphosphate 5 phosphatase OCRL 1; Inositol polyphosphate 5 phosphatase OCRL1; Inositol polyphosphate 5-phosphatase OCRL-1; INPP5F; LOCR; Lowe oculocerebrorenal syndrome protein; NPHL2; OCRL 1; OCRL; OCRL_HUMAN; OCRL1; Oculocerebrorenal syndrome of Lowe; Phosphatidylinositol polyphosphate 5 phosphatase;
免疫原
Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. Expressed in the retina and the retinal pigment epithelium.
- Q01968 OCRL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPLPVGAQPLATVEGMEMKGPLREPCALTLAQRNGQYELIIQLHEKEQHVQDIIPINSHFRCVQEAEETLLIDIASNSGCKIRVQGDWIRERRFEIPDEEHCLKFLSAVLAAQKAQSQLLVPEQKDSSSWYQKLDTKDKPSVFSGLLGFEDNFSSMNLDKKINSQNQPTGIHREPPPPPFSVNKMLPREKEASNKEQPKVTNTMRKLFVPNTQSGQREGLIKHILAKREKEYVNIQTFRFFVGTWNVNGQSPDSGLEPWLNCDPNPPDIYCIGFQELDLSTEAFFYFESVKEQEWSMAVERGLHSKAKYKKVQLVRLVGMMLLIFARKDQCRYIRDIATETVGTGIMGKMGNKGGVAVRFVFHNTTFCIVNSHLAAHVEDFERRNQDYKDICARMSFVVPNQTLPQLNIMKHEVVIWLGDLNYRLCMPDANEVKSLINKKDLQRLLKFDQLNIQRTQKKAFVDFNEGEIKFIPTYKYDSKTDRWDSSGKCRVPAWCDRILWRGTNVNQLNYRSHMELKTSDHKPVSALFHIGVKVVDERRYRKVFEDSVRIMDRMENDFLPSLELSRREFVFENVKFRQLQKEKFQISNNGQVPCHFSFIPKLNDSQYCKPWLRAEPFEGYLEPNETVDISLDVYVSKDSVTILNSGEDKIEDILVLHLDRGKDYFLTISGNYLPSCFGTSLEALCRMKRPIREVPVTKLIDLEEDSFLEKEKSLLQMVPLDEGASERPLQVPKEIWLLVDHLFKYACHQEDLFQTPGMQEELQQIIDCLDTSIPETIPGSNHSVAEALLIFLEALPEPVICYELYQRCLDSAYDPRICRQVISQLPRCHRNVFRYLMAFLRELLKFSEYNSVNANMIATLFTSLLLRPPPNLMARQTPSDRQRAIQFLLGFLLGSEED
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q01968 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K127 | Ubiquitination | Uniprot | |
| Y133 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K478 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| T880 | Phosphorylation | Uniprot | |
| S882 | Phosphorylation | Uniprot |
研究背景
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2. Able also to hydrolyzes the 4-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate. Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes. Involved in primary cilia assembly. Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling.
Cytoplasmic vesicle>Phagosome membrane. Early endosome membrane. Membrane>Clathrin-coated pit. Cell projection>Cilium>Photoreceptor outer segment. Cell projection>Cilium. Cytoplasmic vesicle. Endosome. Golgi apparatus>trans-Golgi network. Lysosome.
Note: Also found on macropinosomes (PubMed:25869668). Colocalized with APPL1 on phagosomes (PubMed:22072788).
Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts. Expressed in the retina and the retinal pigment epithelium.
Interacts with APPL1, FAM109A/SES1 and FAM109B/SES2; APPL1-binding and FAM109A-binding are mutually exclusive. Interacts with clathrin heavy chain. Interacts with several Rab GTPases, at least RAB1B, RAB5A, RAB6A, RAB8A and RAB31; these interactions may play a dual role in targeting OCRL to the specific membranes and stimulating the phosphatase activity. Interaction with RAB8A modulates OCRL recruitment to cilia. Interacts with INPP5F.
The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature.
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.
研究領域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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