OCT1 Antibody - #DF8586

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製品: OCT1 Antibody
カタログ: DF8586
タンパク質の説明: Rabbit polyclonal antibody to OCT1
アプリケーション: WB
反応性: Human, Rat
分子量: 61 kDa; 61kD(Calculated).
ユニプロット: O15245
RRID: AB_2841790

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Polyclonal
特異性:
OCT1 Antibody detects endogenous levels of total OCT1.
RRID:
AB_2841790
引用形式: Affinity Biosciences Cat# DF8586, RRID:AB_2841790.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

hOCT1; OCT1; oct1_cds; Organic cation transporter 1; S22A1_HUMAN; Slc22a1; solute carrier family 22 (organic cation transporter), member 1; Solute carrier family 22 member 1;

免疫原

免疫原:

A synthesized peptide derived from human OCT1, corresponding to a region within N-terminal amino acids.

Uniprot:

O15245(S22A1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A1(6580)
発現特異性:
O15245 S22A1_HUMAN:

Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines.

タンパク質配列:
MPTVDDILEQVGESGWFQKQAFLILCLLSAAFAPICVGIVFLGFTPDHHCQSPGVAELSQRCGWSPAEELNYTVPGLGPAGEAFLGQCRRYEVDWNQSALSCVDPLASLATNRSHLPLGPCQDGWVYDTPGSSIVTEFNLVCADSWKLDLFQSCLNAGFLFGSLGVGYFADRFGRKLCLLGTVLVNAVSGVLMAFSPNYMSMLLFRLLQGLVSKGNWMAGYTLITEFVGSGSRRTVAIMYQMAFTVGLVALTGLAYALPHWRWLQLAVSLPTFLFLLYYWCVPESPRWLLSQKRNTEAIKIMDHIAQKNGKLPPADLKMLSLEEDVTEKLSPSFADLFRTPRLRKRTFILMYLWFTDSVLYQGLILHMGATSGNLYLDFLYSALVEIPGAFIALITIDRVGRIYPMAMSNLLAGAACLVMIFISPDLHWLNIIIMCVGRMGITIAIQMICLVNAELYPTFVRNLGVMVCSSLCDIGGIITPFIVFRLREVWQALPLILFAVLGLLAAGVTLLLPETKGVALPETMKDAENLGRKAKPKENTIYLKVQTSEPSGT

PTMs - O15245 基板として

Site PTM Type Enzyme
S196 Phosphorylation
Y361 Phosphorylation
T541 Phosphorylation

研究背景

機能:

Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.

PTMs:

Phosphorylated.

細胞の位置付け:

Basolateral cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines.

タンパク質ファミリー:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

研究領域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

参考文献

1). CP-25 improves nephropathy in collagen-induced arthritis rats by inhibiting the renal inflammatory response. International Immunopharmacology, 2020 (PubMed: 33182042) [IF=5.6]

Application: WB    Species: rats    Sample: Spleen tissue

Fig. 7. CP-25 upregulated renal OAT1 and OCT1 expression in CIA rats. Expression of the renal transporters OAT1 and OCT1 were measured by western blotting and semi-quantitatively analysed based on the internal reference Na+/K+ ATPase. The data are presented as the mean ± SD, N = 4, ##P < 0.01 vs. the normal group; **P < 0.01 vs. the CIA model group. Bonferroni’s test was used for multiple comparisons between groups.

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