製品: Pepsin A Antibody
カタログ: DF8591
タンパク質の説明: Rabbit polyclonal antibody to Pepsin A
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Xenopus
分子量: 41 kDa; 42kD(Calculated).
ユニプロット: P0DJD7
RRID: AB_2841795

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
Pepsin A Antibody detects endogenous levels of total Pepsin A.
RRID:
AB_2841795
引用形式: Affinity Biosciences Cat# DF8591, RRID:AB_2841795.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Pepsin A 4; pepsin A; pepsinogen 3; pepsinogen 4; pepsinogen 4, group I (pepsinogen A); pepsinogen A4; PGA3; PGA5;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGEAIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNLPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
80
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P0DJD7 基板として

Site PTM Type Enzyme
Y126 Phosphorylation
Y371 Phosphorylation

研究背景

機能:

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the peptidase A1 family.

研究領域

· Organismal Systems > Digestive system > Protein digestion and absorption.

参考文献

1). Pepsin Increases the Proliferation of Vocal Cord Leukoplakia Epithelial Cells by Inducing Autophagy. Otolaryngology–Head and Neck Surgery, 2023 (PubMed: 37264983) [IF=3.4]

2). Pepsin enhances glycolysis to promote malignant transformation of vocal fold leukoplakia epithelial cells with dysplasia. European Archives of Oto-Rhino-Laryngology, 2022 (PubMed: 36380093) [IF=2.6]

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