RAP1 Antibody - #DF8598
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(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8598, RRID:AB_2841802.
折りたたみ／展開
Dopamine receptor interacting protein 5; Dopamine receptor-interacting protein 5; DRIP 5; DRIP5; hRap1; MGC105533; RAP 1; RAP1 homolog; RAP1, yeast, homolog if; RAP1, yeast, homolog of; Repressor/activator protein 1 homolog; TE2IP_HUMAN; Telomeric Repeat Binding Factor 2 Interacting Protein; Telomeric repeat-binding factor 2-interacting protein 1; TERF2-interacting protein; TERF2-interacting telomeric protein 1; TERF2IP; TRF2 Interacting Telomeric Protein RAP1; TRF2 interacting telomeric RAP1 protein; TRF2-interacting telomeric protein 1; TRF2-interacting telomeric protein;
免疫原
- Q9NYB0 TE2IP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEAMDLGKDPNGPTHSSTLFVRDDGSSMSFYVRPSPAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEALAEASGDFISTQYILDCVERNERLELEAYRLGPASAADTGSEAKPGALAEGAAEPEPQRHAGRIAFTDADDVAILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGDAPVSPSSQKLKRKAEEDPEAADSGEPQNKRTPDLPEEEYVKEEIQENEEAVKKMLVEATREFEEVVVDESPPDFEIHITMCDDDPPTPEEDSETQPDEEEEEEEEKVSQPEVGAAIKIIRQLMEKFNLDLSTVTQAFLKNSGELEATSAFLASGQRADGYPIWSRQDDIDLQKDDEDTREALVKKFGAQNVARRIEFRKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NYB0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| C54 | S-Nitrosylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S205 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K208 | Acetylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| Y238 | Phosphorylation | Uniprot | |
| K240 | Sumoylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot |
研究背景
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
Nucleus. Cytoplasm. Chromosome. Chromosome>Telomere.
Note: Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.
Ubiquitous. Highly expressed.
Associates with the I-kappa-B-kinase (IKK) core complex, composed of CHUK, IKBKB and IKBKG (By similarity). Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with TERF2; the interaction is direct. Does not interact with TERF1. Interacts with SLX4/BTBD12.
Belongs to the RAP1 family.
参考文献
Application: WB Species: rat Sample: HEI-OC1 cells
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