製品: SEMA3A Antibody
カタログ: DF8609
タンパク質の説明: Rabbit polyclonal antibody to SEMA3A
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 70~100kDa; 89kD(Calculated).
ユニプロット: Q14563
RRID: AB_2841813

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
SEMA3A Antibody detects endogenous levels of total SEMA3A.
RRID:
AB_2841813
引用形式: Affinity Biosciences Cat# DF8609, RRID:AB_2841813.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Coll 1; Coll1; Collapsin 1; HH16; Hsema I; Hsema III; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Sema III; SEMA1; SEMA1-PEN; SEMA3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A precursor; Semaphorin III; semaphorin L; Semaphorin like growth cone guidance homolog of chick collapsin; Semaphorin-D; Semaphorin3A; SemD;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q14563 SEM3A_HUMAN:

Expressed in the dorsal root ganglia.

タンパク質配列:
MGWLTRIVCLFWGVLLTARANYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRRTRRQDIRNGDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRSV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
91
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14563 基板として

Site PTM Type Enzyme
S42 Phosphorylation
S405 Phosphorylation
Y410 Phosphorylation
S698 Phosphorylation
K727 Ubiquitination

研究背景

機能:

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the dorsal root ganglia.

サブユニット構造:

Interacts with PLXND1.

タンパク質ファミリー:

Strong binding to neuropilin is mediated by the carboxy third of the protein.

Belongs to the semaphorin family.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

参考文献

1). Astragaloside Ⅳ negatively regulates Gpr97-TPL2 signaling to protect against hyperhomocysteine-exacerbated sepsis associated acute kidney injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38237511) [IF=7.9]

2). Luteolin Stimulates Proliferation and Inhibits Late Differentiation of Primary Rat Calvarial Osteoblast Induced by High-dose Dexamethasone via Sema3A /NRP1/Pleixin A1. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2021 (PubMed: 33327910) [IF=2.8]

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