製品: SEMA3F Antibody
カタログ: DF8611
タンパク質の説明: Rabbit polyclonal antibody to SEMA3F
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 88 kDa; 88kD(Calculated).
ユニプロット: Q13275
RRID: AB_2841815

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(83%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SEMA3F Antibody detects endogenous levels of total SEMA3F.
RRID:
AB_2841815
引用形式: Affinity Biosciences Cat# DF8611, RRID:AB_2841815.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

SEM3F_HUMAN; sema domain immunoglobulin domain Ig short basic domain secreted 3F; sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F; Sema III/F; Sema IV; SEMA-IV; SEMA3F; SEMA4; SemaIII/F; SemaIV; SEMAK; Semaphorin IV; Semaphorin-3F; Semaphorin3F; SemaphorinIV;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13275 SEM3F_HUMAN:

Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.

タンパク質配列:
MLVAGLLLWASLLTGAWPSFPTQDHLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILLKDEDHDRMYVGSKDYVLSLDLHDINREPLIIHWAASPQRIEECVLSGKDVNGECGNFVRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQAVRGRGSRATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEELYAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSAERNDDKLYFFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFLKARLVCSVPGEDGIETHFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRGSAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFTPSMKSTKDYPDEVINFMRSHPLMYQAVYPLQRRPLVVRTGAPYRLTTIAVDQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQELEELMLEEVEVFKDPAPVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYCAWDGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVAGSAAFLECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRALQLSDRGLYSCTATENNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPPGAGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPSPREAPGAPRSPEPQDQKKPRNRRHHPPDT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Rabbit
83
Chicken
43
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13275 基板として

Site PTM Type Enzyme
Y131 Phosphorylation
Y144 Phosphorylation
K198 Acetylation
K200 Acetylation
K425 Acetylation
S437 Phosphorylation
Y442 Phosphorylation
Y478 Phosphorylation
T485 Phosphorylation
S766 Phosphorylation

研究背景

機能:

May play a role in cell motility and cell adhesion.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.

タンパク質ファミリー:

Belongs to the semaphorin family.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

参考文献

1). Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35265682) [IF=3.6]

Application: WB    Species: Rat    Sample: hippocampus tissue

Figure 7 Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, non-significant.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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