Synphilin-1 Antibody - #DF8619
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8619, RRID:AB_2841823.
折りたたみ／展開
Alpha synuclein interacting protein; Alpha-synuclein-interacting protein; MGC39814; SNCAIP; SNCAP_HUMAN; Sph1; Synphilin-1; Synphilin1; Synuclein alpha interacting protein (synphilin); Synuclein alpha interacting protein; SYPH 1;
免疫原
Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
- Q9Y6H5 SNCAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAPEYLDLDEIDFSDDISYSVTSLKTIPELCRRCDTQNEDRSVSSSSWNCGISTLITNTQKPTGIADVYSKFRPVKRVSPLKHQPETLENNESDDQKNQKVVEYQKGGESDLGPQPQELGPGDGVGGPPGKSSEPSTSLGELEHYDLDMDEILDVPYIKSSQQLASFTKVTSEKRILGLCTTINGLSGKACSTGSSESSSSNMAPFCVLSPVKSPHLRKASAVIHDQHKLSTEETEISPPLVKCGSAYEPENQSKDFLNKTFSDPHGRKVEKTTPDCQLRAFHLQSSAAESKPEEQVSGLNRTSSQGPEERSEYLKKVKSILNIVKEGQISLLPHLAADNLDKIHDENGNNLLHIAASQGHAECLQHLTSLMGEDCLNERNTEKLTPAGLAIKNGQLECVRWMVSETEAIAELSCSKDFPSLIHYAGCYGQEKILLWLLQFMQEQGISLDEVDQDGNSAVHVASQHGYLGCIQTLVEYGANVTMQNHAGEKPSQSAERQGHTLCSRYLVVVETCMSLASQVVKLTKQLKEQTVERVTLQNQLQQFLEAQKSEGKSLPSSPSSPSSPASRKSQWKSPDADDDSVAKSKPGVQEGIQVLGSLSASSRARPKAKDEDSDKILRQLLGKEISENVCTQEKLSLEFQDAQASSRNSKKIPLEKRELKLARLRQLMQRSLSESDTDSNNSEDPKTTPVRKADRPRPQPIVESVESMDSAESLHLMIKKHTLASGGRRFPFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIPPNQPSGDPQQPSPDSTAAQKVATSPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDKGRTLQRTSTSNESGDQLKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASSASKGKNKAA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6H5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S559 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| T725 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot |
研究背景
Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
Cytoplasm.
Note: Detected in cytoplasmic inclusion bodies, together with SNCA.
Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
Homodimer (Probable). Heterodimer of isoform 1 and isoform 2 (Probable). Interacts with SIAH1, SIAH2, SNCA, RNF19A AND PRKN. Isoform 2 has a strong tendency to form aggregates and can sequester isoform 1.
研究領域
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
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