TRPV4 Antibody - #DF8624

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製品: TRPV4 Antibody
カタログ: DF8624
タンパク質の説明: Rabbit polyclonal antibody to TRPV4
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Sheep, Rabbit
分子量: 98 kDa; 98kD(Calculated).
ユニプロット: Q9HBA0
RRID: AB_2841828

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
TRPV4 Antibody detects endogenous levels of total TRPV4.
RRID:
AB_2841828
引用形式: Affinity Biosciences Cat# DF8624, RRID:AB_2841828.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BCYM3; CMT2C; HMSN2C; osm 9 like TRP channel 4; Osm-9-like TRP channel 4; OSM9 like transient receptor potential channel 4; Osmosensitive transient receptor potential channel 4; OTRPC 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; Transient receptor potential protein 12; TRP 12; TRP12; TRPV 4; TrpV4; TRPV4_HUMAN; Vanilloid receptor like channel 2; Vanilloid receptor like protein 2; Vanilloid receptor related osmotically activated channel; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR 4; VR OAC; VR-OAC; VR4; VRL 2; VRL-2; VRL2; VROAC;

免疫原

免疫原:
Uniprot:

Q9HBA0(TRPV4_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRPV4(59341)
発現特異性:
Q9HBA0 TRPV4_HUMAN:

Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).

タンパク質配列:
MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEASVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANMKVCNEDQTNCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
82
Xenopus
73
Chicken
67
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBA0 基板として

Site PTM Type Enzyme
Y91 Phosphorylation
Y110 Phosphorylation
T112 Phosphorylation
Y113 Phosphorylation
S134 Phosphorylation
S162 Phosphorylation Q05655 (PRKCD)
T175 Phosphorylation Q05655 (PRKCD)
S189 Phosphorylation Q05655 (PRKCD)
Y253 Phosphorylation P07948 (LYN)
S319 Phosphorylation
T335 Phosphorylation
K344 Ubiquitination
Y411 Phosphorylation
S470 Phosphorylation
T505 Phosphorylation
Y508 Phosphorylation
Y805 Phosphorylation
S824 Phosphorylation P17612 (PRKACA)

研究背景

機能:

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage.

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

PTMs:

N-glycosylated.

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein. Cell junction>Adherens junction. Cell projection>Cilium.
Note: Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.

Cell membrane.

Cell membrane.

Endoplasmic reticulum.

Endoplasmic reticulum.

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).

サブユニット構造:

Homotetramer (Probable). Self-associates in an isoform-specific manner. Isoform 1 and isoform 5 can oligomerize, but isoform 2, isoform 4 and isoform 6 cannot oligomerize. Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). Interacts with CTNNB1 (By similarity). The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain) (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1. Interacts with ITPR3. Interacts with AQP5; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (By similarity). Interacts with ANO1 (By similarity). Interacts (via C-terminus) with PKD2 (via C-terminus).

タンパク質ファミリー:

The ANK repeat region mediates interaction with Ca(2+)-calmodulin and ATP binding (By similarity). The ANK repeat region mediates interaction with phosphatidylinositol-4,5-bisphosphate and related phosphatidylinositides (PubMed:25256292).

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

参考文献

1). Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anterior cruciate ligament-transected rat osteoarthritis model. LIFE SCIENCES, 2019 (PubMed: 31055086) [IF=6.1]

Application: WB    Species: rat    Sample: articular cartilage

Fig. 2. |TRPV4 inhibitor inhibited the high expression of calmodulin and cleaved caspase-8. (A) TRPV4, calmodulin,cleaved caspase-8 protein expressions were detected by western blotting
2). Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Detrusor Hyperactivity with Impaired Contractility via Transient Potential Vanilloid Channels: A Rat Model for Ovarian Hormone Deficiency. International journal of molecular sciences, 2024 (PubMed: 38732143) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 4 LiESWT increased neuronal regeneration, synaptic transmission, and receptor response. The expressions of neuronal endogenous markers (NF, NeuN, and GFAP) and muscarinic receptor (M2 and M3), and purinergic receptor (P2X7) markers were assessed by immunostaining (A–H) and Western blots (I,J). Nuclear DNA was labeled with DAPI (blue). (A–D) The M2 immunostaining was markedly expressed in the UL and SL of the (A) sham group. On the contrary, there was less M2 staining expression in the thinner and defective urothelial mucosa in the UL (yellow arrows) of the (B) OVX group, but the immunostainings in the (C) OVX + SW4 group and the (D) OVX + SW8 group were enhanced. (E–H) Double-labeled analysis of M2 (red, upper panels) and NF (green, lower panels) was distributed in the ML of the (E) sham group. However, the double staining of the (G) OVX + SW4 group and the (H) OVX + SW8 group were widely expressed compared with the (F) OVX group. (I,J) Quantifications of the percentage of neurogenesis-related markers, muscarinic receptors, and purinergic receptors were evaluated by Western blotting. The expressions obviously decreased in the OVX group compared with the sham group. However, the expressions significantly increased in the OVX + SW4 group and OVX + SW8 group compared with the OVX group. Therefore, the LiESWT promoted bladder synaptic transmission, receptor response, and neurogenesis to ameliorate the bladder detrusor contractile response. Nuclear DNA was labeled with DAPI (blue). LiESWT, low-intensity extracorporeal shock wave therapy; OVX + SW4, OHD status for 12 months, followed by once weekly LiESWT for 4 weeks; OVX + SW8, OHD status for 12 months, followed by twice weekly LiESWT for 4 weeks; DAPI, 4′,6-diamidino-2-phenylindole; NF, neurofilament; NeuN, neuronal nuclear antigen and neuron; GFAP, glial fibrillary acidic protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; UL, urothelial layer; SL, suburothelial layer. Data are expressed as the means ± SD for n = 8. * p < 0.05 and ** p < 0.01 versus the sham group; ## p < 0.01 versus the OVX group; †† p < 0.01 versus the OVX + SW4 group.

Application: IF/ICC    Species: Rat    Sample:

Figure 4 LiESWT increased neuronal regeneration, synaptic transmission, and receptor response. The expressions of neuronal endogenous markers (NF, NeuN, and GFAP) and muscarinic receptor (M2 and M3), and purinergic receptor (P2X7) markers were assessed by immunostaining (A–H) and Western blots (I,J). Nuclear DNA was labeled with DAPI (blue). (A–D) The M2 immunostaining was markedly expressed in the UL and SL of the (A) sham group. On the contrary, there was less M2 staining expression in the thinner and defective urothelial mucosa in the UL (yellow arrows) of the (B) OVX group, but the immunostainings in the (C) OVX + SW4 group and the (D) OVX + SW8 group were enhanced. (E–H) Double-labeled analysis of M2 (red, upper panels) and NF (green, lower panels) was distributed in the ML of the (E) sham group. However, the double staining of the (G) OVX + SW4 group and the (H) OVX + SW8 group were widely expressed compared with the (F) OVX group. (I,J) Quantifications of the percentage of neurogenesis-related markers, muscarinic receptors, and purinergic receptors were evaluated by Western blotting. The expressions obviously decreased in the OVX group compared with the sham group. However, the expressions significantly increased in the OVX + SW4 group and OVX + SW8 group compared with the OVX group. Therefore, the LiESWT promoted bladder synaptic transmission, receptor response, and neurogenesis to ameliorate the bladder detrusor contractile response. Nuclear DNA was labeled with DAPI (blue). LiESWT, low-intensity extracorporeal shock wave therapy; OVX + SW4, OHD status for 12 months, followed by once weekly LiESWT for 4 weeks; OVX + SW8, OHD status for 12 months, followed by twice weekly LiESWT for 4 weeks; DAPI, 4′,6-diamidino-2-phenylindole; NF, neurofilament; NeuN, neuronal nuclear antigen and neuron; GFAP, glial fibrillary acidic protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; UL, urothelial layer; SL, suburothelial layer. Data are expressed as the means ± SD for n = 8. * p < 0.05 and ** p < 0.01 versus the sham group; ## p < 0.01 versus the OVX group; †† p < 0.01 versus the OVX + SW4 group.
3). Treadmill exercise decreases cerebral edema in rats with local cerebral infarction by modulating AQP4 polar expression through the caveolin-1/TRPV4 signaling pathway. BRAIN RESEARCH BULLETIN, 2022 (PubMed: 35961528) [IF=3.8]
4). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.6]

Application: IF/ICC    Species: Human    Sample: AS fibroblasts

Fig. 4 Expression of TRPC1 and TRPV4 in AS fibroblasts after different treatment. A Protein expressions of TRPC1 and TRPV4 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of protein expression. C Representative images showing immunofluorescence staining of TRPC1. Magnification, × 400. D Representative images showing immunofluorescence staining of TRPV4. Magnification, × 400. E Mean fluorescence intensity of TRPC1. F Mean fluorescence intensity of TRPV4. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group

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