ZO-2 Antibody - #DF8632

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製品: ZO-2 Antibody
カタログ: DF8632
タンパク質の説明: Rabbit polyclonal antibody to ZO-2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 133 kDa; 134kD(Calculated).
ユニプロット: Q9UDY2
RRID: AB_2841836

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
ZO-2 Antibody detects endogenous levels of total ZO-2.
RRID:
AB_2841836
引用形式: Affinity Biosciences Cat# DF8632, RRID:AB_2841836.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); MGC26306; PFIC4; Tight junction protein 2; Tight junction protein ZO 2; Tight junction protein ZO-2; TJP2; X104; ZO 2; ZO-2; ZO2; ZO2_HUMAN; Zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2;

免疫原

免疫原:
Uniprot:

Q9UDY2(ZO2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TJP2(9414)
発現特異性:
Q9UDY2 ZO2_HUMAN:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

タンパク質配列:
MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGASTKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKMDHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
92
Dog
92
Chicken
92
Rabbit
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UDY2 基板として

Site PTM Type Enzyme
S16 Phosphorylation
T91 Phosphorylation
S130 Phosphorylation
S150 Phosphorylation
S153 Phosphorylation
S156 Phosphorylation
S163 Phosphorylation
S168 Phosphorylation
S170 Phosphorylation
S174 Phosphorylation
S186 Phosphorylation
S192 Phosphorylation
S196 Phosphorylation
S200 Phosphorylation
T212 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
S232 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
Y249 Phosphorylation
Y253 Phosphorylation
Y257 Phosphorylation
Y261 Phosphorylation
Y265 Phosphorylation
S266 Phosphorylation
Y269 Phosphorylation
S279 Phosphorylation
S284 Phosphorylation
S286 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
S296 Phosphorylation
Y319 Phosphorylation
S325 Phosphorylation
T333 Phosphorylation
T335 Phosphorylation
T339 Phosphorylation
S385 Phosphorylation
S394 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
S406 Phosphorylation
S413 Phosphorylation
S415 Phosphorylation
Y423 Phosphorylation
S424 Phosphorylation
Y426 Phosphorylation
Y428 Phosphorylation
S430 Phosphorylation
S431 Phosphorylation
S432 Phosphorylation
S440 Phosphorylation
S441 Phosphorylation
T445 Phosphorylation
S450 Phosphorylation
T455 Phosphorylation
T457 Phosphorylation
K460 Methylation
S461 Phosphorylation
T462 Phosphorylation
T468 Phosphorylation
K475 Ubiquitination
Y479 Phosphorylation
T494 Phosphorylation
S499 Phosphorylation
Y506 Phosphorylation
T510 Phosphorylation
S520 Phosphorylation
Y574 Phosphorylation
T585 Phosphorylation
S590 Phosphorylation
S606 Phosphorylation
Y638 Phosphorylation
S702 Phosphorylation
T707 Phosphorylation
S711 Phosphorylation
S713 Phosphorylation
K730 Methylation
T760 Phosphorylation
S767 Phosphorylation
K769 Ubiquitination
S770 Phosphorylation
T771 Phosphorylation
T778 Phosphorylation
T831 Phosphorylation
S832 Phosphorylation
S836 Phosphorylation
S886 Phosphorylation
S902 Phosphorylation
Y903 Phosphorylation
T905 Phosphorylation
Y911 Phosphorylation
S913 Phosphorylation
S916 Phosphorylation
S920 Phosphorylation
T925 Phosphorylation
Y932 Phosphorylation
T933 Phosphorylation
S953 Phosphorylation
S961 Phosphorylation
S966 Phosphorylation
S978 Phosphorylation
S979 Phosphorylation
S986 Phosphorylation
S1006 Phosphorylation
Y1007 Phosphorylation
S1012 Phosphorylation
Y1013 Phosphorylation
Y1015 Phosphorylation
K1016 Ubiquitination
T1027 Phosphorylation
S1031 Phosphorylation
S1048 Phosphorylation
S1053 Phosphorylation
T1054 Phosphorylation
S1067 Phosphorylation
S1068 Phosphorylation
Y1118 Phosphorylation
T1124 Phosphorylation
T1131 Phosphorylation
T1135 Phosphorylation
S1137 Phosphorylation
S1147 Phosphorylation
Y1150 Phosphorylation
S1156 Phosphorylation
Y1157 Phosphorylation
S1159 Phosphorylation
Y1166 Phosphorylation
S1171 Phosphorylation
S1174 Phosphorylation
R1176 Methylation
Y1178 Phosphorylation
Y1179 Phosphorylation
S1182 Phosphorylation

研究背景

機能:

Plays a role in tight junctions and adherens junctions.

細胞の位置付け:

Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

サブユニット構造:

Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus. Interacts with SCRIB. Interacts with USP53 (via the C-terminal region) (By similarity).

タンパク質ファミリー:

Belongs to the MAGUK family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

参考文献

1). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 31863867) [IF=9.3]

Application: WB    Species: Mice    Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.
2). A novel nano material for anti-cerebral ischemia: Preparation and Application of Borneol Angelica Polysaccharide Liposomes. JOURNAL OF LIPOSOME RESEARCH, 2023 (PubMed: 35875973) [IF=4.4]
3). Preparation of Borneol Angelica Polysaccharide Liposomes and Study Evaluation of Its Anti-Cerebral Ischemia-Reperfusion Inflammatory Reaction. Research Square, 2021

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