FZD5 Antibody - #DF8658

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製品: FZD5 Antibody
カタログ: DF8658
タンパク質の説明: Rabbit polyclonal antibody to FZD5
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 64 kDa; 65kD(Calculated).
ユニプロット: Q13467
RRID: AB_2841862

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(89%), Sheep(89%), Rabbit(89%), Dog(89%)
クローナリティ:
Polyclonal
特異性:
FZD5 Antibody detects endogenous levels of total FZD5.
RRID:
AB_2841862
引用形式: Affinity Biosciences Cat# DF8658, RRID:AB_2841862.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C2orf31; DKFZP434E2135; Frizzled 5 seven transmembrane spanning receptor; Frizzled class receptor 5; Frizzled family receptor 5; Frizzled homolog 5; Frizzled-5; Frizzled5; Fz 5; Fz-5; Fz5; FZD 5; fzd5; FZD5_HUMAN; FzE 5; FzE5; HFZ 5; hFz5; MGC129692; Seven transmembrane receptor frizzled 5; Uncharacterized protein C2orf31; Wnt receptor; Frizzled 8 seven transmembrane spanning receptor; frizzled 8, seven transmembrane spanning receptor; Frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled homolog 8; Frizzled-8; FZ 8; Fz-8; FZ8; FZD 8; FZD8; FZD8_HUMAN; hFZ 8; hFz8; HGNC4046; Homolog of Drosophila Frizzled 8;

免疫原

免疫原:
Uniprot:

Q13467(FZD5_HUMAN) >>Visit HPA database.

遺伝子(ID):
FZD5(7855)
タンパク質配列:
MARPDPSAPPSLLLLLLAQLVGRAAAASKAPVCQEITVPMCRGIGYNLTHMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPERMSCDRLPVLGRDAEVLCMDYNRSEATTAPPRPFPAKPTLPGPPGAPASGGECPAGGPFVCKCREPFVPILKESHPLYNKVRTGQVPNCAVPCYQPSFSADERTFATFWIGLWSVLCFISTSTTVATFLIDMERFRYPERPIIFLSACYLCVSLGFLVRLVVGHASVACSREHNHIHYETTGPALCTIVFLLVYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYAQYFHLAAWLIPSVKSITALALSSVDGDPVAGICYVGNQNLNSLRGFVLGPLVLYLLVGTLFLLAGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGIFTLLYTVPASIVVACYLYEQHYRESWEAALTCACPGHDTGQPRAKPEYWVLMLKYFMCLVVGITSGVWIWSGKTVESWRRFTSRCCCRPRRGHKSGGAMAAGDYPEASAALTGRTGPPGPAATYHKQVSLSHV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Bovine
89
Sheep
89
Dog
89
Rabbit
89
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13467 基板として

Site PTM Type Enzyme
Ubiquitination
S7 Phosphorylation
S11 Phosphorylation
N47 N-Glycosylation
T440 Phosphorylation
Y556 Phosphorylation
T575 Phosphorylation
Y576 Phosphorylation
S581 Phosphorylation

研究背景

機能:

Receptor for Wnt proteins. Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (By similarity). In neurons, activation of WNT7A promotes formation of synapses. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity).

PTMs:

Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cell junction>Synapse. Perikaryon. Cell projection>Dendrite. Cell projection>Axon.
Note: Localized at the plasma membrane and also found at the Golgi.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Binding of unsaturated fatty acid molecules (via FZ domain) promotes homodimerization. Interacts with WNT2B. Interacts with WNT7A. Interacts with GOPC (By similarity).

タンパク質ファミリー:

The PDZ-binding motif mediates interaction with GOPC.

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

The FZ domain is involved in binding with Wnt ligands.

Belongs to the G-protein coupled receptor Fz/Smo family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

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