GAS6 Antibody - #DF8659

Figure 4 GAS6 is highly expressed and plays an important role in TIC maintenance in ICC. (A) Circle plots show interaction strength and number of interactions in cell-cell communication among 6 major clusters (left) using CellChat and between epithelial and other cells (right). Line width correlates with communication probability. (B) Dot plots depict the significance (–log10 P value) and strength (log2 mean value) of detailed ligand-receptor pairs between epithelial and other cell types analyzed by CellphoneDB. (C) Violin plot displays the Gas6 expression differences between WNThi and WNTlo epithelial cell subclusters. (D) Violin plots show Axl and Mertk expression in MoMϕDC subclusters. (E–H) Flow plots (E) show Tom+GAS6+ tumor-infiltrating cells and the levels of MRC1, MERTK, and AXL expression in infiltrating Reg-TAMs. Graphs depict statistical analysis of GAS6+ in Tom+/– cells in mouse ICC (F) as well as AXL+ (G) and MERTK+ (H) expression in MRC1+/– cells in ICC. (I) Treatment strategy with anti-GAS6/IgG in ICC mice. (J) Representative liver morphology image for survival outcome analysis after anti-GAS6 treatment. (K) Kaplan-Meier OS curve for ICC mice after anti-GAS6 treatment. (L) Representative liver morphology image after 6 weeks of anti-GAS6 treatment (left) and statistical analysis of liver-to-body weight ratio (right). (M) Fluorescence images of lineage tracing (left) (scale bar: 50 μm). Statistical analysis of Tom+ cell numbers (middle), and Tom+ cell area (right) after treatment. (N–S) Representative images of GAS6 staining in human ICC tissue from SYSUCC cohort 1 (N) (scale bar: 200 μm) and FAHSYSU cohort 2 (Q) (scale bar: 100 μm). Kaplan-Meier curves based on GAS6 expression in ICC: OS and DFS for SYSUCC cohort 1 (O and P), and OS and RFS for FAHSYSU cohort 2 (R and S). Data represent the mean ± SD (F–H, L, and M). P value were calculated by 2-tailed,, unpaired Student’s t test (C, F–H, L, and M), log-rank test (K, O, P, R, and S).