GIT1 Antibody - #DF8660
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8660, RRID:AB_2841864.
折りたたみ／展開
ARF GAP GIT1; ARF GTPase activating protein GIT1; ARF GTPase-activating protein GIT1; CAT 1; CAT-1; CaT1; Cool associated and tyrosine phosphorylated protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein coupled receptor kinase interacting ArfGAP 1; G protein coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GIT1_HUMAN; GRK interacting protein 1; GRK-interacting protein 1;
免疫原
- Q9Y2X7 GIT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2X7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S36 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | O94806 (PRKD3) | Uniprot |
| K49 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Q05397 (PTK2) , P12931 (SRC) | Uniprot |
| K358 | Ubiquitination | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| Y383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| Y510 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| K517 | Methylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| T537 | Phosphorylation | Uniprot | |
| Y545 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y554 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| T601 | Phosphorylation | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| C674 | S-Nitrosylation | Uniprot | |
| K677 | Acetylation | Uniprot | |
| S700 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| T753 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).
Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).
Cytoplasm.
Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1 (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. Interacts with SCRIB. Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct. Found in a complex with ENTR1, PTPN13 and GIT1. Interacts with YWHAZ (By similarity).
The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.