HCN2 Antibody - #DF8663
| 製品: | HCN2 Antibody |
| カタログ: | DF8663 |
| タンパク質の説明: | Rabbit polyclonal antibody to HCN2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Dog, Chicken |
| 分子量: | 96 kDa; 97kD(Calculated). |
| ユニプロット: | Q9UL51 |
| RRID: | AB_2841867 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8663, RRID:AB_2841867.
折りたたみ／展開
BCNG-2; BCNG2; Brain cyclic nucleotide gated channel 2; Brain cyclic nucleotide-gated channel 2; HAC1; Hcn2; HCN2_HUMAN; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2;
免疫原
- Q9UL51 HCN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UL51 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S81 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y476 | Phosphorylation | P12931 (SRC) | Uniprot |
| T576 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Q13237 (PRKG2) | Uniprot |
| Y697 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S786 | Phosphorylation | Uniprot | |
| T792 | Phosphorylation | Uniprot | |
| Y795 | Phosphorylation | Uniprot | |
| T851 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| S868 | Phosphorylation | Uniprot |
研究背景
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
Phosphorylation at Ser-668 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating.
Cell membrane>Multi-pass membrane protein.
Highly expressed throughout the brain. Detected at low levels in heart.
The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Heterotetramer with HCN1. Forms an obligate 4:4 complex with accessory subunit PEX5L. Interacts with KCNE2 (By similarity). Homotetramer.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Belongs to the potassium channel HCN family.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
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