RAB35 Antibody - #DF8710
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8710, RRID:AB_2841914.
折りたたみ/展開
GTP binding protein RAY; GTP-binding protein RAY; H ray; OTTHUMP00000240256; OTTHUMP00000240257; OTTHUMP00000240258; OTTHUMP00000240259; RAB1C; Rab35; RAB35 member RAS oncogene family; RAB35, member RAS oncogene family; RAB35_HUMAN; Ras related protein rab 1c (GTP binding protein ray); Ras related protein Rab 1C; Ras related protein Rab35; Ras-related protein Rab-1C; Ras-related protein Rab-35; RAY;
免疫原
- Q15286 RAB35_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15286 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y5 | Phosphorylation | Uniprot | |
S17 | Phosphorylation | Uniprot | |
K21 | Ubiquitination | Uniprot | |
S36 | Phosphorylation | Uniprot | |
Y37 | Phosphorylation | Uniprot | |
T39 | Phosphorylation | Uniprot | |
T49 | Phosphorylation | Uniprot | |
K56 | Ubiquitination | Uniprot | |
K58 | Ubiquitination | Uniprot | |
T64 | Phosphorylation | Uniprot | |
T72 | Phosphorylation | Uniprot | |
T74 | Phosphorylation | Uniprot | |
S75 | Phosphorylation | Uniprot | |
T76 | Phosphorylation | Uniprot | |
Y77 | Phosphorylation | Uniprot | |
K121 | Ubiquitination | Uniprot | |
K128 | Ubiquitination | Uniprot | |
Y136 | Phosphorylation | Uniprot | |
K137 | Ubiquitination | Uniprot | |
K178 | Ubiquitination | Uniprot | |
K189 | Ubiquitination | Uniprot |
研究背景
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active.
Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A.
Cell membrane>Lipid-anchor>Cytoplasmic side. Membrane>Clathrin-coated pit. Cytoplasmic vesicle>Clathrin-coated vesicle. Endosome. Melanosome.
Note: Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065).
Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner. Interacts with DENND1C; weak interaction which is nucleotide-independent. Interacts (GTP-bound form) with ACAP2 and MICALL1; the interaction is direct and probably recruits ACAP2 and MICALL1 to membranes. Interacts with EHD1; the interaction is indirect through MICALL1 and probably recruits EHD1 to membranes (By similarity). Interacts with GDI1, GDI2, CHM and CHML; phosphorylation at Thr-72 disrupts these interactions.
Belongs to the small GTPase superfamily. Rab family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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