Phospho-CD28 (Tyr218) Antibody - #DF8735
| 製品: | Phospho-CD28 (Tyr218) Antibody |
| カタログ: | DF8735 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CD28 (Tyr218) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 25 kDa; 25kD(Calculated). |
| ユニプロット: | P10747 |
| RRID: | AB_2841939 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8735, RRID:AB_2841939.
折りたたみ／展開
CD 28; CD28; CD28 antigen; CD28 molecule; CD28_HUMAN; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; TP44;
免疫原
- P10747 CD28_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P10747 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N37 | N-Glycosylation | Uniprot | |
| N71 | N-Glycosylation | Uniprot | |
| N105 | N-Glycosylation | Uniprot | |
| N129 | N-Glycosylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| Y191 | Phosphorylation | P06239 (LCK) , Q08881 (ITK) , P06241 (FYN) | Uniprot |
| T195 | Phosphorylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| Y209 | Phosphorylation | Q08881 (ITK) | Uniprot |
| Y218 | Phosphorylation | Q08881 (ITK) | Uniprot |
| S220 | Phosphorylation | Uniprot |
研究背景
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation. Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.
CD40LG induces tyrosine phosphorylation of isoform 3.
Membrane>Single-pass type I membrane protein.
Cell surface.
Expressed in T-cells and plasma cells, but not in less mature B-cells.
Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2. Isoform 3 interacts with CD40LG.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Immune diseases > Autoimmune thyroid disease.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Human Diseases > Immune diseases > Allograft rejection.
· Human Diseases > Immune diseases > Graft-versus-host disease.
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Intestinal immune network for IgA production. (View pathway)
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For Research Use Only.
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