MT-ND3 Antibody - #DF8747

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製品: MT-ND3 Antibody
カタログ: DF8747
タンパク質の説明: Rabbit polyclonal antibody to MT-ND3
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 13 kDa; 13kD(Calculated).
ユニプロット: P03897
RRID: AB_2841951

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
MT-ND3 Antibody detects endogenous levels of total MT-ND3.
RRID:
AB_2841951
引用形式: Affinity Biosciences Cat# DF8747, RRID:AB_2841951.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EC=1.6.5.3; Mitochondrially encoded NADH dehydrogenase 3; MT-ND3; MTND3; NADH dehydrogenase subunit 3; NADH dehydrogenase, subunit 3 (complex I); NADH-ubiquinone oxidoreductase chain 3; ND3; NU3M_HUMAN;

免疫原

免疫原:
Uniprot:

P03897(NU3M_HUMAN) >>Visit HPA database.

遺伝子(ID):
MT-ND3(4537)
タンパク質配列:
MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSLAYEWLQKGLDWTE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P03897 基板として

Site PTM Type Enzyme
S52 Phosphorylation
Y104 Phosphorylation

研究背景

機能:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

細胞の位置付け:

Mitochondrion membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the complex I subunit 3 family.

研究領域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

参考文献

1). Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain. CARCINOGENESIS, 2020 (PubMed: 31628462) [IF=4.7]

Application: WB    Species: mouse    Sample: HCC and adjacent normal tissues

Figure 1. |Mitochondrial genome and mitomiR expression profile in HCC and adjacent normal tissues. (A) Heatmap displaying mitochondrial genome transcripts. (B) qRT-PCR analysis of mitochondrially transcribed mRNAs. 12S rRNA served as the internal control to normalize the data. *P < 0.05 was considered statistically significant. (C) Western blotting of mt-ND1, mt-ND5, mt-ND6, mt-CYB, mt-CO2 and mt-ATP8. VADC1/Porin served as the internal control. *P < 0.05 was considered statistically significant.

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