Dystrophin Antibody - #DF8748
| 製品: | Dystrophin Antibody |
| カタログ: | DF8748 |
| タンパク質の説明: | Rabbit polyclonal antibody to Dystrophin |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Dog, Chicken |
| 分子量: | 427kDa; 427kD(Calculated). |
| ユニプロット: | P11532 |
| RRID: | AB_2841952 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8748, RRID:AB_2841952.
折りたたみ／展開
BMD; CMD3B; DMD; DMD_HUMAN; Duchenne muscular dystrophy protein; Dystrophin; Muscular dystrophy Duchenne and Becker types;
免疫原
Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells.
- P11532 DMD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLWWEEVEDCYEREDVQKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQNNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIARYQLGIEKLLDPEDVDTTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPPKVTKEEHFQLHHQMHYSQQITVSLAQGYERTSSPKPRFKSYAYTQAAYVTTSDPTRSPFPSQHLEAPEDKSFGSSLMESEVNLDRYQTALEEVLSWLLSAEDTLQAQGEISNDVEVVKDQFHTHEGYMMDLTAHQGRVGNILQLGSKLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHRVLMDLQNQKLKELNDWLTKTEERTRKMEEEPLGPDLEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRLTEEQCLFSAWLSEKEDAVNKIHTTGFKDQNEMLSSLQKLAVLKADLEKKKQSMGKLYSLKQDLLSTLKNKSVTQKTEAWLDNFARCWDNLVQKLEKSTAQISQAVTTTQPSLTQTTVMETVTTVTTREQILVKHAQEELPPPPPQKKRQITVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIFRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIIAFYNQLQQLEQMTTTAENWLKIQPTTPSEPTAIKSQLKICKDEVNRLSDLQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFKQVFSDVQAREKELQTIFDTLPPMRYQETMSAIRTWVQQSETKLSIPQLSVTDYEIMEQRLGELQALQSSLQEQQSGLYYLSTTVKEMSKKAPSEISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMNKLRKIQNHIQTLKKWMAEVDVFLKEEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKNEAEPEFASRLETELKELNTQWDHMCQQVYARKEALKGGLEKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQKAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPVAQEALKKELETLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVEFKLKTTENIPGGAEEISEVLDSLENLMRHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQETEKSLHLIQESLTFIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKEAAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNVLTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSITEVGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADTLLDESEKKKPQQKEDVLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKLVEPQISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMNEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVREETMMVMTEDMPLEISYVPSTYLTEITHVSQALLEVEQLLNAPDLCAKDFEDLFKQEESLKNIKDSLQQSSGRIDIIHSKKTAALQSATPVERVKLQEALSQLDFQWEKVNKMYKDRQGRFDRSVEKWRRFHYDIKIFNQWLTEAEQFLRKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRTLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLSDRKKRLEEQKNILSEFQRDLNEFVLWLEEADNIASIPLEPGKEQQLKEKLEQVKLLVEELPLRQGILKQLNETGGPVLVSAPISPEEQDKLENKLKQTNLQWIKVSRALPEKQGEIEAQIKDLGQLEKKLEDLEEQLNHLLLWLSPIRNQLEIYNQPNQEGPFDVKETEIAVQAKQPDVEEILSKGQHLYKEKPATQPVKRKLEDLSSEWKAVNRLLQELRAKQPDLAPGLTTIGASPTQTVTLVTQPVVTKETAISKLEMPSSLMLEVPALADFNRAWTELTDWLSLLDQVIKSQRVMVGDLEDINEMIIKQKATMQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQNQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEGPYTVDAIQKKITETKQLAKDLRQWQTNVDVANDLALKLLRDYSADDTRKVHMITENINASWRSIHKRVSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATRKERLLEDSKGVKELMKQWQDLQGEIEAHTDVYHNLDENSQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVNDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P11532 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Acetylation | Uniprot | |
| K225 | Acetylation | Uniprot | |
| T289 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| K487 | Acetylation | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| T671 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| T960 | Phosphorylation | Uniprot | |
| S988 | Phosphorylation | Uniprot | |
| S1012 | Phosphorylation | Uniprot | |
| K1017 | Acetylation | Uniprot | |
| S1081 | Phosphorylation | Uniprot | |
| S1096 | Phosphorylation | Uniprot | |
| T1136 | Phosphorylation | Uniprot | |
| S1161 | Phosphorylation | Uniprot | |
| K1201 | Acetylation | Uniprot | |
| K1288 | Ubiquitination | Uniprot | |
| S1442 | Phosphorylation | Uniprot | |
| S1455 | Phosphorylation | Uniprot | |
| Y1509 | Phosphorylation | Uniprot | |
| S1517 | Phosphorylation | Uniprot | |
| T1525 | Phosphorylation | Uniprot | |
| Y1552 | Phosphorylation | Uniprot | |
| T1590 | Phosphorylation | Uniprot | |
| K1644 | Ubiquitination | Uniprot | |
| T1647 | Phosphorylation | Uniprot | |
| S1658 | Phosphorylation | Uniprot | |
| K1911 | Acetylation | Uniprot | |
| S2037 | Phosphorylation | Uniprot | |
| T2060 | Phosphorylation | Uniprot | |
| S2079 | Phosphorylation | Uniprot | |
| S2200 | Phosphorylation | Uniprot | |
| S2280 | Phosphorylation | Uniprot | |
| S2306 | Phosphorylation | Uniprot | |
| Y2651 | Phosphorylation | Uniprot | |
| S2652 | Phosphorylation | Uniprot | |
| T2656 | Phosphorylation | Uniprot | |
| S2669 | Phosphorylation | Uniprot | |
| S2672 | Phosphorylation | Uniprot | |
| S2728 | Phosphorylation | Uniprot | |
| K2808 | Acetylation | Uniprot | |
| T2868 | Phosphorylation | Uniprot | |
| Y2885 | Phosphorylation | Uniprot | |
| T2913 | Phosphorylation | Uniprot | |
| S2993 | Phosphorylation | Uniprot | |
| S3066 | Phosphorylation | Uniprot | |
| T3081 | Phosphorylation | Uniprot | |
| S3291 | Phosphorylation | Uniprot | |
| K3308 | Ubiquitination | Uniprot | |
| K3312 | Ubiquitination | Uniprot | |
| K3317 | Ubiquitination | Uniprot | |
| K3351 | Ubiquitination | Uniprot | |
| Y3354 | Phosphorylation | Uniprot | |
| Y3359 | Phosphorylation | Uniprot | |
| T3361 | Phosphorylation | Uniprot | |
| K3374 | Ubiquitination | Uniprot | |
| Y3394 | Phosphorylation | Uniprot | |
| T3399 | Phosphorylation | Uniprot | |
| T3408 | Phosphorylation | Uniprot | |
| S3420 | Phosphorylation | Uniprot | |
| S3424 | Phosphorylation | Uniprot | |
| S3425 | Phosphorylation | Uniprot | |
| S3429 | Phosphorylation | Uniprot | |
| T3433 | Phosphorylation | Uniprot | |
| S3435 | Phosphorylation | Uniprot | |
| S3483 | Phosphorylation | Uniprot | |
| S3490 | Phosphorylation | Uniprot | |
| S3500 | Phosphorylation | Uniprot | |
| Y3524 | Phosphorylation | Uniprot | |
| S3537 | Phosphorylation | Uniprot | |
| S3541 | Phosphorylation | Uniprot | |
| T3548 | Phosphorylation | Uniprot | |
| S3549 | Phosphorylation | Uniprot | |
| S3552 | Phosphorylation | Uniprot | |
| K3563 | Ubiquitination | Uniprot | |
| T3610 | Phosphorylation | Uniprot | |
| S3612 | Phosphorylation | Uniprot | |
| S3613 | Phosphorylation | Uniprot | |
| S3615 | Phosphorylation | Uniprot | |
| T3616 | Phosphorylation | Uniprot | |
| S3617 | Phosphorylation | Uniprot | |
| S3621 | Phosphorylation | Uniprot | |
| S3623 | Phosphorylation | Uniprot | |
| S3624 | Phosphorylation | Uniprot | |
| T3652 | Phosphorylation | Uniprot | |
| S3666 | Phosphorylation | Uniprot | |
| T3675 | Phosphorylation | Uniprot | |
| T3684 | Phosphorylation | Uniprot |
研究背景
Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton. Cell junction>Synapse>Postsynaptic cell membrane.
Note: In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs). In adult muscle, NMJ localization depends upon ANK2 presence, but not in newborn animals.
Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells.
Interacts with SYNM (By similarity). Interacts with the syntrophins SNTA1, SNTB1, SNTB2, SNTG1 and SNTG2. Interacts with KRT19. Component of the dystrophin-associated glycoprotein complex which is composed of three subcomplexes: a cytoplasmic complex comprised of DMD (or UTRN), DTNA and a number of syntrophins, such as SNTB1, SNTB2, SNTG1 and SNTG2, the transmembrane dystroglycan complex, and the sarcoglycan-sarcospan complex. Interacts with DAG1 (betaDAG1) with DMD; the interaction is inhibited by phosphorylation on the PPXY motif of DAG1. Interacts with CMYA5 (By similarity). Directly interacts with ANK2 and ANK3; these interactions do not interfere with betaDAG1-binding and are necessary for proper localization in muscle cells (By similarity). Identified in a dystroglycan complex that contains at least PRX, DRP2, UTRN, DMD and DAG1 (By similarity).
研究領域
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
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