p63 MAPK Antibody - #DF8796
| 製品: | p63 MAPK Antibody |
| カタログ: | DF8796 |
| タンパク質の説明: | Rabbit polyclonal antibody to p63 MAPK |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 66 kDa; 66kD(Calculated). |
| ユニプロット: | P31152 |
| RRID: | AB_2841994 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8796, RRID:AB_2841994.
折りたたみ／展開
ERK-4; ERK3; Erk3 related; ERK4; Extracellular signal regulated kinase 4; Extracellular signal-regulated kinase 4; MAP kinase 4; MAP kinase isoform p63; MAPK 4; MAPK4; Mitogen activated protein kinase 4; Mitogen-activated protein kinase 4; MK04_HUMAN; p63 MAPK; p63-MAPK; p63MAPK; PRKM4; Protein kinase, mitogen-activated, 4; Protein-tyrosine kinase ERK3-related; RMNK2;
免疫原
- P31152 MK04_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAVDSRACRKVAVKKIALSDARSMKHALREIKIIRRLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYIHSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLFLEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNKLGDLNGACIPEHPGDLVQTEAFSKERW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31152 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Q8IW41 (MAPKAPK5) , Q13177 (PAK2) | Uniprot |
| S196 | Phosphorylation | P31152 (MAPK4) | Uniprot |
| Y206 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y424 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| T472 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot |
PTMs - P31152 酵素として
| Substrate | Site | Source |
|---|---|---|
| P31152 (MAPK4) | S196 | Uniprot |
| Q8IW41 (MAPKAPK5) | T182 | Uniprot |
研究背景
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity).
Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following interaction with MAPKAPK5.
High expression in heart and brain.
Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity).
The FRIEDE motif is required for docking MAPKAPK5.
In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究領域
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
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