hnRNP E1 Antibody - #DF8831
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8831, RRID:AB_2842028.
折りたたみ／展開
Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; hnRNP E1; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX; nucleic acid binding protein sub 2.3; Nucleic acid- binding protein SUB2.3; Nucleic acid-binding protein SUB2.3; PCBP1; PCBP1_HUMAN; poly(rC) binding protein 1; Poly(rC)-binding protein 1; rCbinding protein 1;
免疫原
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
- Q15365 PCBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15365 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Sumoylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Sumoylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K37 | Acetylation | Uniprot | |
| S43 | Phosphorylation | P31751 (AKT2) , P31749 (AKT1) | Uniprot |
| S50 | Phosphorylation | Uniprot | |
| C54 | S-Nitrosylation | Uniprot | |
| T60 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| T99 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| C109 | S-Nitrosylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K115 | Sumoylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T127 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| C163 | S-Nitrosylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| C194 | S-Nitrosylation | Uniprot | |
| C201 | S-Nitrosylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| K268 | Sumoylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| R306 | Methylation | Uniprot | |
| K314 | Sumoylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T330 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| K351 | Acetylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| C355 | S-Nitrosylation | Uniprot | |
| S356 | Phosphorylation | Uniprot |
研究背景
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.
Phosphorylated; lowers poly(rC)-binding activity.
Nucleus. Cytoplasm.
Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
研究領域
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Genetic Information Processing > Transcription > Spliceosome.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.