AGK Antibody - #DF8835
| 製品: | AGK Antibody |
| カタログ: | DF8835 |
| タンパク質の説明: | Rabbit polyclonal antibody to AGK |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Bovine, Dog, Chicken |
| 分子量: | 47 kDa; 47kD(Calculated). |
| ユニプロット: | Q53H12 |
| RRID: | AB_2842032 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8835, RRID:AB_2842032.
折りたたみ／展開
2610037M15Rik; 6720408I04Rik; Acylglycerol kinase mitochondrial; Acylglycerol kinase, mitochondrial; agk; AGK_HUMAN; AI465370; FLJ10842; hAGK; HsMuLK; MuLK; Multi substrate lipid kinase; Multi-substrate lipid kinase; Multiple substrate lipid kinase; RGD1562046;
免疫原
- Q53H12 AGK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTVFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGSRKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSPTQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q53H12 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Acetylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| C43 | S-Nitrosylation | Uniprot | |
| C72 | S-Nitrosylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y226 | Phosphorylation | Uniprot | |
| K231 | Acetylation | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot |
研究背景
Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex. The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
Mitochondrion inner membrane>Peripheral membrane protein. Mitochondrion intermembrane space.
Note: Localizes in the mitochondrion intermembrane space, where it associates with the inner membrane (PubMed:28712724). It is unclear whether the N-terminal hydrophobic region forms a transmembrane region or associates with the membrane without crossing it (PubMed:28712724, PubMed:28712726).
Highly expressed in muscle, heart, kidney and brain.
Component of the TIM22 complex, which core is composed of TIMM22, associated with TIMM10 (TIMM10A and/or TIMM10B), TIMM9, AGK and TIMM29.
Belongs to the AGK family.
研究領域
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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