GTF2H4 Antibody - #DF8857
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8857, RRID:AB_2842054.
折りたたみ／展開
Basic transcription factor 2 52 kDa subunit; Basic transcription factor 52 kDa subunit; BTF2 p52; General transcription factor IIH polypeptide 4 52kDa; General transcription factor IIH polypeptide 4 52kDa subunit; General transcription factor IIH polypeptide 4 52kDa variant; General transcription factor IIH polypeptide 4; General transcription factor IIH subunit 4; Gtf2h4; OTTHUMP00000029049; OTTHUMP00000164874; P52; TF2H4_HUMAN; TFB2; TFIIH basal transcription factor complex p52 subunit; TFIIH protein; TIFIIH; Transcription factor II H; Transcription factor IIH 52 kDa subunit;
免疫原
- Q92759 TF2H4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESTPSRGLNRVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVSGAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92759 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S27 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot |
研究背景
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Nucleus.
Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.
Belongs to the TFB2 family.
研究領域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
Restrictive clause
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For Research Use Only.
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