NCOR2 Antibody - #DF8896

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製品: NCOR2 Antibody
カタログ: DF8896
タンパク質の説明: Rabbit polyclonal antibody to NCOR2
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
分子量: 274 kDa; 274kD(Calculated).
ユニプロット: Q9Y618
RRID: AB_2842092

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
NCOR2 Antibody detects endogenous levels of total NCOR2.
RRID:
AB_2842092
引用形式: Affinity Biosciences Cat# DF8896, RRID:AB_2842092.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CTG 26; CTG repeat protein 26; CTG26; N CoR2; N-CoR2; NCoR 2; Ncor2; NCOR2_HUMAN; Nuclear Receptor Co-Repressor 2; Nuclear receptor corepressor 2; retinoic-acid-receptor-associated corepressor; Silencing Mediator for Retanoid and Thyroid Hormone Receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP 270; SMAP270; SMRT; SMRTE; SMRTE tau; T3 receptor associating factor; T3 receptor-associating factor; Thyroid retinoic acid receptor associated corepressor; Thyroid-; TNRC 14; TNRC14; TRAC 1; TRAC; TRAC1;

免疫原

免疫原:
Uniprot:

Q9Y618(NCOR2_HUMAN) >>Visit HPA database.

遺伝子(ID):
NCOR2(9612)
発現特異性:
Q9Y618 NCOR2_HUMAN:

Ubiquitous. High levels of expression are detected in lung, spleen and brain.

タンパク質配列:
MSGSTQPVAQTWRATEPRYPPHSLSYPVQIARTHTDVGLLEYQHHSRDYASHLSPGSIIQPQRRRPSLLSEFQPGNERSQELHLRPESHSYLPELGKSEMEFIESKRPRLELLPDPLLRPSPLLATGQPAGSEDLTKDRSLTGKLEPVSPPSPPHTDPELELVPPRLSKEELIQNMDRVDREITMVEQQISKLKKKQQQLEEEAAKPPEPEKPVSPPPIESKHRSLVQIIYDENRKKAEAAHRILEGLGPQVELPLYNQPSDTRQYHENIKINQAMRKKLILYFKRRNHARKQWEQKFCQRYDQLMEAWEKKVERIENNPRRRAKESKVREYYEKQFPEIRKQRELQERMQSRVGQRGSGLSMSAARSEHEVSEIIDGLSEQENLEKQMRQLAVIPPMLYDADQQRIKFINMNGLMADPMKVYKDRQVMNMWSEQEKETFREKFMQHPKNFGLIASFLERKTVAECVLYYYLTKKNENYKSLVRRSYRRRGKSQQQQQQQQQQQQQQQQQPMPRSSQEEKDEKEKEKEAEKEEEKPEVENDKEDLLKEKTDDTSGEDNDEKEAVASKGRKTANSQGRRKGRITRSMANEANSEEAITPQQSAELASMELNESSRWTEEEMETAKKGLLEHGRNWSAIARMVGSKTVSQCKNFYFNYKKRQNLDEILQQHKLKMEKERNARRKKKKAPAAASEEAAFPPVVEDEEMEASGVSGNEEEMVEEAEALHASGNEVPRGECSGPATVNNSSDTESIPSPHTEAAKDTGQNGPKPPATLGADGPPPGPPTPPPEDIPAPTEPTPASEATGAPTPPPAPPSPSAPPPVVPKEEKEEETAAAPPVEEGEEQKPPAAEELAVDTGKAEEPVKSECTEEAEEGPAKGKDAEAAEATAEGALKAEKKEGGSGRATTAKSSGAPQDSDSSATCSADEVDEAEGGDKNRLLSPRPSLLTPTGDPRANASPQKPLDLKQLKQRAAAIPPIQVTKVHEPPREDAAPTKPAPPAPPPPQNLQPESDAPQQPGSSPRGKSRSPAPPADKEAFAAEAQKLPGDPPCWTSGLPFPVPPREVIKASPHAPDPSAFSYAPPGHPLPLGLHDTARPVLPRPPTISNPPPLISSAKHPSVLERQIGAISQGMSVQLHVPYSEHAKAPVGPVTMGLPLPMDPKKLAPFSGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSYVEAQEDYLRREAKLLKREGTPPPPPPSRDLTEAYKTQALGPLKLKPAHEGLVATVKEAGRSIHEIPREELRHTPELPLAPRPLKEGSITQGTPLKYDTGASTTGSKKHDVRSLIGSPGRTFPPVHPLDVMADARALERACYEESLKSRPGTASSSGGSIARGAPVIVPELGKPRQSPLTYEDHGAPFAGHLPRGSPVTTREPTPRLQEGSLSSSKASQDRKLTSTPREIAKSPHSTVPEHHPHPISPYEHLLRGVSGVDLYRSHIPLAFDPTSIPRGIPLDAAAAYYLPRHLAPNPTYPHLYPPYLIRGYPDTAALENRQTIINDYITSQQMHHNAATAMAQRADMLRGLSPRESSLALNYAAGPRGIIDLSQVPHLPVLVPPTPGTPATAMDRLAYLPTAPQPFSSRHSSSPLSPGGPTHLTKPTTTSSSERERDRDRERDRDREREKSILTSTTTVEHAPIWRPGTEQSSGSSGGGGGSSSRPASHSHAHQHSPISPRTQDALQQRPSVLHNTGMKGIITAVEPSTPTVLRSTSTSSPVRPAATFPPATHCPLGGTLDGVYPTLMEPVLLPKEAPRVARPERPRADTGHAFLAKPPARSGLEPASSPSKGSEPRPLVPPVSGHATIARTPAKNLAPHHASPDPPAPPASASDPHREKTQSKPFSIQELELRSLGYHGSSYSPEGVEPVSPVSSPSLTHDKGLPKHLEELDKSHLEGELRPKQPGPVKLGGEAAHLPHLRPLPESQPSSSPLLQTAPGVKGHQRVVTLAQHISEVITQDYTRHHPQQLSAPLPAPLYSFPGASCPVLDLRRPPSDLYLPPPDHGAPARGSPHSEGGKRSPEPNKTSVLGGGEDGIEPVSPPEGMTEPGHSRSAVYPLLYRDGEQTEPSRMGSKSPGNTSQPPAFFSKLTESNSAMVKSKKQEINKKLNTHNRNEPEYNISQPGTEIFNMPAITGTGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGPLAGPHHAWDEEPKPLLCSQYETLSDSE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Xenopus
92
Zebrafish
82
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y618 基板として

Site PTM Type Enzyme
S51 Phosphorylation
S54 Phosphorylation
S67 Phosphorylation
S79 Phosphorylation
K106 Ubiquitination
S121 Phosphorylation
K137 Ubiquitination
S149 Phosphorylation
S152 Phosphorylation
T156 Phosphorylation
S215 Phosphorylation
S221 Phosphorylation
K271 Ubiquitination
Y332 Phosphorylation
Y333 Phosphorylation
K335 Ubiquitination
S373 Phosphorylation
S380 Phosphorylation
S493 Phosphorylation
T550 Phosphorylation
T553 Phosphorylation
S554 Phosphorylation
S708 Phosphorylation
S711 Phosphorylation
T741 Phosphorylation
S745 Phosphorylation
S746 Phosphorylation
T748 Phosphorylation
S750 Phosphorylation
S753 Phosphorylation
T756 Phosphorylation
T803 Phosphorylation
T807 Phosphorylation
S814 Phosphorylation
S816 Phosphorylation
K844 Sumoylation
K863 Sumoylation
K878 Acetylation
K892 Sumoylation
S908 Phosphorylation
S922 Phosphorylation
S939 Phosphorylation
S943 Phosphorylation
T946 Phosphorylation
S956 Phosphorylation
K959 Acetylation
T979 Phosphorylation
S1017 Phosphorylation
S1018 Phosphorylation
S1023 Phosphorylation
S1025 Phosphorylation
S1126 Phosphorylation
K1160 Ubiquitination
K1168 Sumoylation
S1173 Phosphorylation
T1198 Phosphorylation
S1202 Phosphorylation
S1207 Phosphorylation
K1210 Acetylation
K1210 Ubiquitination
S1219 Phosphorylation
R1226 Methylation
T1233 Phosphorylation
K1240 Acetylation
K1240 Sumoylation
S1251 Phosphorylation
S1253 Phosphorylation
S1262 Phosphorylation
Y1270 Phosphorylation
K1273 Ubiquitination
S1298 Phosphorylation
T1304 Phosphorylation
K1308 Ubiquitination
S1323 Phosphorylation
S1324 Phosphorylation
S1326 Phosphorylation
S1341 Phosphorylation
R1352 Methylation
Y1370 Phosphorylation
T1383 Phosphorylation
S1390 Phosphorylation
K1408 Sumoylation
K1408 Ubiquitination
S1424 Phosphorylation
T1436 Phosphorylation
S1450 Phosphorylation
T1452 Phosphorylation
T1455 Phosphorylation
K1458 Sumoylation
K1458 Ubiquitination
S1464 Phosphorylation
S1475 Phosphorylation
S1479 Phosphorylation
Y1504 Phosphorylation
K1509 Ubiquitination
T1514 Phosphorylation
S1518 Phosphorylation
K1535 Acetylation
K1535 Sumoylation
S1539 Phosphorylation
T1542 Phosphorylation
R1556 Methylation
S1558 Phosphorylation
T1561 O-Glycosylation
T1562 O-Glycosylation
R1563 Methylation
T1566 Phosphorylation
R1568 Methylation
S1573 Phosphorylation
S1576 Phosphorylation
K1578 Ubiquitination
K1584 Sumoylation
T1588 Phosphorylation
S1595 Phosphorylation
T1599 Phosphorylation
S1609 Phosphorylation
R1616 Methylation
S1619 Phosphorylation
R1639 Methylation
R1653 Methylation
Y1661 Phosphorylation
R1671 Methylation
R1682 Methylation
R1711 Methylation
R1716 Methylation
R1729 Methylation
T1750 Phosphorylation
S1774 Phosphorylation
S1775 Phosphorylation
S1778 Phosphorylation
T1783 Phosphorylation
T1786 Phosphorylation
K1787 Acetylation
S1794 Phosphorylation
S1813 Phosphorylation
T1816 Phosphorylation
T1819 Phosphorylation
S1852 Phosphorylation
S1858 Phosphorylation
S1861 Phosphorylation
S1890 Phosphorylation
T1891 Phosphorylation
T1893 Phosphorylation
T1952 Phosphorylation
K1959 Acetylation
S1964 Phosphorylation
S1970 Phosphorylation
S1971 Phosphorylation
S1973 Phosphorylation
S1976 Phosphorylation
S1986 Phosphorylation
S2005 Phosphorylation
K2022 Acetylation
K2026 Acetylation
K2026 Sumoylation
K2026 Ubiquitination
S2037 Phosphorylation
Y2040 Phosphorylation
S2043 Phosphorylation
S2044 Phosphorylation
S2046 Phosphorylation
S2054 Phosphorylation
S2057 Phosphorylation
S2058 Phosphorylation
S2060 Phosphorylation
T2062 Phosphorylation
K2069 Sumoylation
K2069 Ubiquitination
K2076 Ubiquitination
S2077 Phosphorylation
K2086 Ubiquitination
S2112 Phosphorylation
S2114 Phosphorylation
Y2161 Phosphorylation
S2162 Phosphorylation
S2178 Phosphorylation
S2194 Phosphorylation
S2197 Phosphorylation
S2203 Phosphorylation
S2223 Phosphorylation
T2229 Phosphorylation
S2236 Phosphorylation
Y2239 Phosphorylation
S2256 Phosphorylation
S2258 Phosphorylation
T2262 Phosphorylation
S2263 Phosphorylation
S2275 Phosphorylation
K2281 Sumoylation
S2393 Phosphorylation
S2407 Phosphorylation
S2413 Phosphorylation
S2420 Phosphorylation
S2426 Phosphorylation
S2432 Phosphorylation
T2440 Phosphorylation
S2452 Phosphorylation
S2453 Phosphorylation
S2456 Phosphorylation
T2457 Phosphorylation
S2475 Phosphorylation
S2511 Phosphorylation
S2513 Phosphorylation

研究背景

機能:

Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. High levels of expression are detected in lung, spleen and brain.

サブユニット構造:

Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. Interacts directly with RARA in the absence of ligand; the interaction represses RARA activity. Interacts (isoform SMRT) with HDAC10. Interacts with MINT. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with CBFA2T3 and ATXN1L. Interacts with RARB; the interaction is weak and does not repress RARB transactivational activity. Interacts with HDAC7 and C1D. Interacts with NR4A2; this interaction increases in the absence of PITX3. Interacts with BCL6 (via the BTB domain), required for BCL6 transcriptional repressor activity on a subset of target genes. Forms ternary complexes with BCOR and BCL6 on target gene promoters but, on enhancer elements, interacts with BCL6 and HDAC3 to repress proximal gene expression. May interact with DEAF1. Interacts with RXRA. Interacts with MECP2 (By similarity). Interacts with ZBTB7A. Interacts with AR. Interacts with TBL1Y.

タンパク質ファミリー:

The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).

The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.

Belongs to the N-CoR nuclear receptor corepressors family.

研究領域

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

参考文献

1). DNA Methylation-Mediated GPX4 Transcriptional Repression and Osteoblast Ferroptosis Promote Titanium Particle-Induced Osteolysis. Research (Washington, D.C.), 2024 (PubMed: 39161535) [IF=8.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. DNMT aberration-induced GPX4 suppression is co-regulated by KLF5. (A) Schematic diagram depicting mouse Gpx4 promoter luciferase reporter, Gpx4p-luc, the KLF5 binding motif, and its position relative to the transcription starting site. (B) Western blot. Calvarial tissues treated with control and TP (20 mg per mouse, 2 weeks) were assayed for KLF5, NCoR, SnoN, and SMRT, with β-actin serving as control. Two random samples per group were shown. Quantification beside blots was presented as mean ± SEM; n = 6; *P < 0.05, Student’s t test. (C) ChIP. Sham or TP-treated calvaria treated with or without SGI-1027 (2.5 mg/kg daily) were immunoprecipitated first with antibodies to KLF5, SMRT, NCoR, or SnoN, and then the immunoprecipitated DNA fragments were amplified through PCR with primer set specific for the KLF5 motif-containing region of the Gpx4 promoter, respectively. The non-immunoprecipitated DNA served as control (Input). PCR products were resolved on agarose gels. Quantifications on the right side were presented as mean ± SEM; n = 6; *P < 0.05, 2-way ANOVA. (D) Western blot. Primary osteoblasts (OBs) treated without or with TP (100 μg/ml) and the KLF5 inhibitor ML264 (10 μM) for 48 h were assayed for GPX4, with β-actin serving as control. Quantification below was presented as mean ± SD of 3 replicated experiments. *P < 0.05, 2-way ANOVA. (E) Luciferase assay. Primary OBs were transfected with a mouse Gpx4 promoter-luciferase reporter (Gpx4p-luc) and a Renilla luciferase reporter control, and then treated without or with TP (100 μg/ml) and SGI-1027 (10 μM) in the absence or presence of ML264 (10 μM) for 48 h. Luciferase activities of the Gpx4 promoter reporter were adjusted to Renilla luciferase activities and presented as box-and-whisker plots of 4 independent experiments. *P < 0.05, 3-way ANOVA.

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