CISH Antibody - #DF8914
| 製品: | CISH Antibody |
| カタログ: | DF8914 |
| タンパク質の説明: | Rabbit polyclonal antibody to CISH |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Sheep, Rabbit, Dog |
| 分子量: | 29kDa; 29kD(Calculated). |
| ユニプロット: | Q9NSE2 |
| RRID: | AB_2842110 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8914, RRID:AB_2842110.
折りたたみ／展開
BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling;
免疫原
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
- Q9NSE2 CISH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NSE2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S142 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot |
研究背景
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR).
研究領域
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
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