ARRS Antibody - #DF8919

(1)  
製品: ARRS Antibody
カタログ: DF8919
タンパク質の説明: Rabbit polyclonal antibody to ARRS
アプリケーション: WB IHC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 45 kDa; 45kD(Calculated).
ユニプロット: P10523
RRID: AB_2842115

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(82%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
ARRS Antibody detects endogenous levels of total ARRS.
RRID:
AB_2842115
引用形式: Affinity Biosciences Cat# DF8919, RRID:AB_2842115.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

48 kDa protein; Arrestin 1; Arrestin; ARRS_HUMAN; Retinal S antigen (48 KDa protein); Retinal S-antigen; Rod photoreceptor arrestin; RP47; S antigen; S antigen retina and pineal gland (arrestin); S arrestin; S-AG; S-arrestin; SAG;

免疫原

免疫原:
Uniprot:

P10523(ARRS_HUMAN) >>Visit HPA database.

遺伝子(ID):
SAG(6295)
発現特異性:
P10523 ARRS_HUMAN:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

タンパク質配列:
MAASGKTSKSEPNHVIFKKISRDKSVTIYLGNRDYIDHVSQVQPVDGVVLVDPDLVKGKKVYVTLTCAFRYGQEDIDVIGLTFRRDLYFSRVQVYPPVGAASTPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGKSCGVDFEVKAFATDSTDAEEDKIPKKSSVRLLIRKVQHAPLEMGPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQVANVVLYSSDYYVKPVAMEEAQEKVPPNSTLTKTLTLLPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVLGILVSYQIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Pig
82
Dog
82
Xenopus
80
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10523 基板として

Site PTM Type Enzyme
K9 Acetylation
Y29 Phosphorylation
Y62 Phosphorylation
T64 Phosphorylation
T66 Phosphorylation
Y120 Phosphorylation
Y129 Phosphorylation
S143 Phosphorylation
Y254 Phosphorylation
Y258 Phosphorylation
S348 Phosphorylation
S349 Phosphorylation
T353 Phosphorylation

研究背景

機能:

Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.

細胞の位置付け:

Cell projection>Cilium>Photoreceptor outer segment. Membrane>Peripheral membrane protein.
Note: Highly expressed in photoreceptor outer segments in light-exposed retina. Evenly distributed throughout rod photoreceptor cells in dark-adapted retina (By similarity). Predominantly dectected at the proximal region of photoreceptor outer segments, near disk membranes (PubMed:3720866).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level).

サブユニット構造:

Monomer. Homodimer. Homotetramer. Interacts with RHO (via the phosphorylated C-terminus).

タンパク質ファミリー:

The C-terminus interferes with binding to non-phosphorylated RHO. Interaction with phosphorylated RHO triggers displacement of the C-terminus and leads to a conformation change that mediates high-affinity RHO binding.

Belongs to the arrestin family.

研究領域

· Organismal Systems > Sensory system > Phototransduction.

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