HspB4 Antibody - #DF8933
製品: | HspB4 Antibody |
カタログ: | DF8933 |
タンパク質の説明: | Rabbit polyclonal antibody to HspB4 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 20 kDa; 20kD(Calculated). |
ユニプロット: | P02489 |
RRID: | AB_2842129 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8933, RRID:AB_2842129.
折りたたみ/展開
Acry 1; Alpha crystallin A chain; Alpha-crystallin A chain; CRYA 1; CRYA1; CRYAA; CRYAA_HUMAN; Crystallin alpha 1; Crystallin alpha A; Heat shock protein beta 4; Heat shock protein beta-4; HSPB 4; HspB4; short form; Zonular Central Nuclear Cataract;
免疫原
- P02489 CRYAA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVTIQHPWFKRTLGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVQDDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFCGPKIQTGLDATHAERAIPVSREEKPTSAPSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02489 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
T13 | Phosphorylation | Uniprot | |
Y18 | Phosphorylation | Uniprot | |
S20 | Phosphorylation | Uniprot | |
R21 | Methylation | Uniprot | |
S45 | Phosphorylation | Uniprot | |
Y48 | Phosphorylation | Uniprot | |
K70 | Acetylation | Uniprot | |
K78 | Acetylation | Uniprot | |
K88 | Acetylation | Uniprot | |
K88 | Methylation | Uniprot | |
K99 | Acetylation | Uniprot | |
S122 | Phosphorylation | Uniprot | |
T140 | Phosphorylation | Uniprot | |
K145 | Acetylation | Uniprot | |
T148 | Phosphorylation | Uniprot | |
S162 | O-Glycosylation | Uniprot |
研究背景
Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA.
O-glycosylated; contains N-acetylglucosamine side chains.
Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
Acetylation at Lys-70 seems to increase chaperone activity.
Undergoes age-dependent proteolytical cleavage at the C-terminus. Alpha-crystallin A(1-172) is the most predominant form produced most rapidly during the first 12 years of age and after this age is present in approximately 50% of the lens molecules.
Cytoplasm. Nucleus.
Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Expressed in eye lens.
Heteropolymer composed of three CRYAA and one CRYAB subunits. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Can also form homodimers and higher homooligomers (via C-terminus). Part of a complex required for lens intermediate filament formation composed of BFSP1, BFSP2 and CRYAA.
Belongs to the small heat shock protein (HSP20) family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.