製品: HspB4 Antibody
カタログ: DF8933
タンパク質の説明: Rabbit polyclonal antibody to HspB4
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 20 kDa; 20kD(Calculated).
ユニプロット: P02489
RRID: AB_2842129

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
HspB4 Antibody detects endogenous levels of total HspB4.
RRID:
AB_2842129
引用形式: Affinity Biosciences Cat# DF8933, RRID:AB_2842129.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Acry 1; Alpha crystallin A chain; Alpha-crystallin A chain; CRYA 1; CRYA1; CRYAA; CRYAA_HUMAN; Crystallin alpha 1; Crystallin alpha A; Heat shock protein beta 4; Heat shock protein beta-4; HSPB 4; HspB4; short form; Zonular Central Nuclear Cataract;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P02489 CRYAA_HUMAN:

Expressed in eye lens.

タンパク質配列:
MDVTIQHPWFKRTLGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVQDDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFCGPKIQTGLDATHAERAIPVSREEKPTSAPSS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02489 基板として

Site PTM Type Enzyme
M1 Acetylation
T13 Phosphorylation
Y18 Phosphorylation
S20 Phosphorylation
R21 Methylation
S45 Phosphorylation
Y48 Phosphorylation
K70 Acetylation
K78 Acetylation
K88 Acetylation
K88 Methylation
K99 Acetylation
S122 Phosphorylation
T140 Phosphorylation
K145 Acetylation
T148 Phosphorylation
S162 O-Glycosylation

研究背景

機能:

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA.

PTMs:

O-glycosylated; contains N-acetylglucosamine side chains.

Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.

Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.

Acetylation at Lys-70 seems to increase chaperone activity.

Undergoes age-dependent proteolytical cleavage at the C-terminus. Alpha-crystallin A(1-172) is the most predominant form produced most rapidly during the first 12 years of age and after this age is present in approximately 50% of the lens molecules.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in eye lens.

サブユニット構造:

Heteropolymer composed of three CRYAA and one CRYAB subunits. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Can also form homodimers and higher homooligomers (via C-terminus). Part of a complex required for lens intermediate filament formation composed of BFSP1, BFSP2 and CRYAA.

タンパク質ファミリー:

Belongs to the small heat shock protein (HSP20) family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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