製品: DNAJA1 Antibody
カタログ: DF8940
タンパク質の説明: Rabbit polyclonal antibody to DNAJA1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 44 kDa; 45kD(Calculated).
ユニプロット: P31689
RRID: AB_2842136

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
DNAJA1 Antibody detects endogenous levels of total DNAJA1.
RRID:
AB_2842136
引用形式: Affinity Biosciences Cat# DF8940, RRID:AB_2842136.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DJ 2; DJ2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; Dnaja1; DNJA1_HUMAN; hDJ 2; HDJ-2; HDJ2; Heat shock 40 kDa protein 4; heat shock protein DNAJ like 2; Heat shock protein J2; HSDJ; HSJ-2; HSJ2; HSPF4; Human DnaJ protein 2; NEDD7; Neural precursor cell expressed developmentally down regulated 7; OTTHUMP00000021193;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P31689 DNJA1_HUMAN:

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.

タンパク質配列:
MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
92
Xenopus
75
Chicken
58
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31689 基板として

Site PTM Type Enzyme
K3 Ubiquitination
Y7 Phosphorylation
Y8 Phosphorylation
K14 Ubiquitination
K23 Acetylation
K23 Ubiquitination
K24 Ubiquitination
K32 Acetylation
K32 Ubiquitination
K37 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K46 Ubiquitination
S49 Phosphorylation
Y52 Phosphorylation
S56 Phosphorylation
K59 Ubiquitination
K60 Ubiquitination
K66 Acetylation
K66 Ubiquitination
S83 Phosphorylation
K105 Ubiquitination
S112 Phosphorylation
T114 Phosphorylation
Y119 Phosphorylation
K125 Ubiquitination
K130 Ubiquitination
K136 Acetylation
K136 Ubiquitination
K143 Ubiquitination
K144 Ubiquitination
T156 Phosphorylation
S175 Phosphorylation
S188 Phosphorylation
K206 Ubiquitination
K214 Ubiquitination
K246 Ubiquitination
R253 Methylation
S278 Phosphorylation
S289 Phosphorylation
K296 Ubiquitination
K301 Ubiquitination
C302 S-Nitrosylation
Y311 Phosphorylation
S335 Phosphorylation
K338 Ubiquitination
S340 Phosphorylation
K344 Ubiquitination
K350 Ubiquitination
Y376 Phosphorylation
Y381 Phosphorylation

研究背景

機能:

Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.

細胞の位置付け:

Membrane>Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm>Perinuclear region. Mitochondrion.
Note: Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.

サブユニット構造:

Identified in a complex with HSPA1B and BAX. Interacts with RNF207.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.