DNAJA1 Antibody - #DF8940
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8940, RRID:AB_2842136.
折りたたみ/展開
DJ 2; DJ2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; Dnaja1; DNJA1_HUMAN; hDJ 2; HDJ-2; HDJ2; Heat shock 40 kDa protein 4; heat shock protein DNAJ like 2; Heat shock protein J2; HSDJ; HSJ-2; HSJ2; HSPF4; Human DnaJ protein 2; NEDD7; Neural precursor cell expressed developmentally down regulated 7; OTTHUMP00000021193;
免疫原
Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
- P31689 DNJA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31689 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K3 | Ubiquitination | Uniprot | |
Y7 | Phosphorylation | Uniprot | |
Y8 | Phosphorylation | Uniprot | |
K14 | Ubiquitination | Uniprot | |
K23 | Acetylation | Uniprot | |
K23 | Ubiquitination | Uniprot | |
K24 | Ubiquitination | Uniprot | |
K32 | Acetylation | Uniprot | |
K32 | Ubiquitination | Uniprot | |
K37 | Ubiquitination | Uniprot | |
K44 | Acetylation | Uniprot | |
K44 | Ubiquitination | Uniprot | |
K46 | Ubiquitination | Uniprot | |
S49 | Phosphorylation | Uniprot | |
Y52 | Phosphorylation | Uniprot | |
S56 | Phosphorylation | Uniprot | |
K59 | Ubiquitination | Uniprot | |
K60 | Ubiquitination | Uniprot | |
K66 | Acetylation | Uniprot | |
K66 | Ubiquitination | Uniprot | |
S83 | Phosphorylation | Uniprot | |
K105 | Ubiquitination | Uniprot | |
S112 | Phosphorylation | Uniprot | |
T114 | Phosphorylation | Uniprot | |
Y119 | Phosphorylation | Uniprot | |
K125 | Ubiquitination | Uniprot | |
K130 | Ubiquitination | Uniprot | |
K136 | Acetylation | Uniprot | |
K136 | Ubiquitination | Uniprot | |
K143 | Ubiquitination | Uniprot | |
K144 | Ubiquitination | Uniprot | |
T156 | Phosphorylation | Uniprot | |
S175 | Phosphorylation | Uniprot | |
S188 | Phosphorylation | Uniprot | |
K206 | Ubiquitination | Uniprot | |
K214 | Ubiquitination | Uniprot | |
K246 | Ubiquitination | Uniprot | |
R253 | Methylation | Uniprot | |
S278 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
K296 | Ubiquitination | Uniprot | |
K301 | Ubiquitination | Uniprot | |
C302 | S-Nitrosylation | Uniprot | |
Y311 | Phosphorylation | Uniprot | |
S335 | Phosphorylation | Uniprot | |
K338 | Ubiquitination | Uniprot | |
S340 | Phosphorylation | Uniprot | |
K344 | Ubiquitination | Uniprot | |
K350 | Ubiquitination | Uniprot | |
Y376 | Phosphorylation | Uniprot | |
Y381 | Phosphorylation | Uniprot |
研究背景
Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Membrane>Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm>Perinuclear region. Mitochondrion.
Note: Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.
Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Identified in a complex with HSPA1B and BAX. Interacts with RNF207.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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