GTSE1 Antibody - #DF8953
(1)
(5)
製品説明
ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
GTSE1 Antibody detects endogenous levels of total GTSE1.
RRID:
AB_2842149
引用形式: Affinity Biosciences Cat# DF8953, RRID:AB_2842149.
引用形式: Affinity Biosciences Cat# DF8953, RRID:AB_2842149.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
B99; B99 homolog; G 2 and S phase expressed 1; G two S phase expressed protein 1; GTSE 1;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q9NYZ3 GTSE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDPKKEDILLLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNVPAAGSHLGQGKRAIPVPNKLGLKKTLLKAPGSTSNLARKSSSGPVWSGASSACTSPAVGKAKSSEFASIPANSSRPLSNISKSGRMGPAMLRPALPAGPVGASSWQAKRVDVSELAAEQLTAPPSASPTQPQTPEGGGQWLNSSCAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSAWRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF
PTMs - Q9NYZ3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Sumoylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K123 | Sumoylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| R182 | Methylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K215 | Methylation | Uniprot | |
| S223 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S228 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K310 | Methylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K331 | Methylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S439 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S464 | Phosphorylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| T489 | Phosphorylation | Uniprot | |
| R492 | Methylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| T513 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| T529 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| T656 | Phosphorylation | Uniprot | |
| T675 | Phosphorylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot |
研究背景
機能:
May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.
PTMs:
Phosphorylated in mitosis.
細胞の位置付け:
Cytoplasm>Cytoskeleton.
Note: Associated with microtubules.
研究領域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
参考文献
1). miR-181a-5p restrains non-small cell lung cancer cell invasion and migration by regulating the GTSE1/p53/NF-κB axis. CELLS TISSUES ORGANS, 2022
(PubMed: 35700709)
[IF=2.7]
Application: WB Species: Human Sample: NSCLC cells
Figure 1 miR-181a-5p was down-regulated and GTSE1 was up-regulated in NSCLC cells. A: expression of miR-181a5p detected by RT-qPCR; B: expression of GTSE1 detected by RT-qPCR; C: protein expression of GTSE1 detected by Western blot. Cell experiment was replicated 3 times. Data were described as mean ± standard deviation. Data comparisons between two groups were analyzed using the t test. The post hoc test was conducted using Tukey’s multiple comparisons test.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.