GTSE1 Antibody - #DF8953

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製品: GTSE1 Antibody
カタログ: DF8953
タンパク質の説明: Rabbit polyclonal antibody to GTSE1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse
分子量: 77 kDa; 77kD(Calculated).
ユニプロット: Q9NYZ3
RRID: AB_2842149

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
GTSE1 Antibody detects endogenous levels of total GTSE1.
RRID:
AB_2842149
引用形式: Affinity Biosciences Cat# DF8953, RRID:AB_2842149.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B99; B99 homolog; G 2 and S phase expressed 1; G two S phase expressed protein 1; GTSE 1;

免疫原

免疫原:

A synthesized peptide derived from human GTSE1, corresponding to a region within the internal amino acids.

Uniprot:

Q9NYZ3(GTSE1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GTSE1(51512)
タンパク質配列:
MDDPKKEDILLLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNVPAAGSHLGQGKRAIPVPNKLGLKKTLLKAPGSTSNLARKSSSGPVWSGASSACTSPAVGKAKSSEFASIPANSSRPLSNISKSGRMGPAMLRPALPAGPVGASSWQAKRVDVSELAAEQLTAPPSASPTQPQTPEGGGQWLNSSCAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSAWRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF

研究背景

機能:

May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.

PTMs:

Phosphorylated in mitosis.

細胞の位置付け:

Cytoplasm>Cytoskeleton.
Note: Associated with microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

参考文献

1). miR-181a-5p restrains non-small cell lung cancer cell invasion and migration by regulating the GTSE1/p53/NF-κB axis. CELLS TISSUES ORGANS, 2022 (PubMed: 35700709) [IF=2.9]

Application: WB    Species: Human    Sample: NSCLC cells

Figure 1 miR-181a-5p was down-regulated and GTSE1 was up-regulated in NSCLC cells. A: expression of miR-181a5p detected by RT-qPCR; B: expression of GTSE1 detected by RT-qPCR; C: protein expression of GTSE1 detected by Western blot. Cell experiment was replicated 3 times. Data were described as mean ± standard deviation. Data comparisons between two groups were analyzed using the t test. The post hoc test was conducted using Tukey’s multiple comparisons test.

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