ROBLD3 Antibody - #DF9021
| 製品: | ROBLD3 Antibody |
| カタログ: | DF9021 |
| タンパク質の説明: | Rabbit polyclonal antibody to ROBLD3 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 14 kDa; 14kD(Calculated). |
| ユニプロット: | Q9Y2Q5 |
| RRID: | AB_2842217 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9021, RRID:AB_2842217.
折りたたみ／展開
ENDAP; Endosomal adaptor protein p14; HSPC003; LAMTOR2; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Late endosomal/lysosomal Mp1 interacting protein; Late endosomal/lysosomal Mp1-interacting protein; LTOR2_HUMAN; MAPBPIP; MAPKSP1 adaptor protein; MAPKSP1AP; Mitogen activated protein binding protein interacting protein; Mitogen-activated protein-binding protein-interacting protein; p14; Ragulator complex protein LAMTOR2; Ragulator2; Roadblock domain containing 3; Roadblock domain containing protein 3; Roadblock domain-containing protein 3; ROBLD 3; RP11 336K24.9;
免疫原
- Q9Y2Q5 LTOR2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQVAAS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2Q5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K70 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S125 | Phosphorylation | Uniprot |
研究背景
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side.
Part of the Ragulator complex composed of LAMTOR1, LAMTOR2, LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer. The Ragulator complex interacts with both the mTORC1 complex and heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; regulated by amino acid availability. The Ragulator complex interacts with SLC38A9; the probable amino acid sensor. Interacts with LAMTOR1 and LAMTOR3; the interaction is direct. Interacts with MAPK1 and MAP2K1 (By similarity).
Belongs to the GAMAD family.
研究領域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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