MYLK2 Antibody - #DF9023

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製品: MYLK2 Antibody
カタログ: DF9023
タンパク質の説明: Rabbit polyclonal antibody to MYLK2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 65 kDa; 65kD(Calculated).
ユニプロット: Q9H1R3
RRID: AB_2842219

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
MYLK2 Antibody detects endogenous levels of total MYLK2.
RRID:
AB_2842219
引用形式: Affinity Biosciences Cat# DF9023, RRID:AB_2842219.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KMLC; MLCK; MLCK, skeletal/cardiac; MLCK2; MYLK 2; MYLK2; MYLK2_HUMAN; Myosin light chain kinase 2; Myosin light chain kinase 2 skeletal muscle; Myosin light chain kinase 2 skeletal/cardiac muscle; MYOSIN LIGHT POLYPEPTIDE KINASE; Myosin light polypeptide kinase, skeletal/cardiac; Skeletal muscle myosin light chain kinase; Skeletal myosin light chain kinase; skeletal/cardiac muscle; skMLCK;

免疫原

免疫原:
Uniprot:

Q9H1R3(MYLK2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MYLK2(85366)
発現特異性:
Q9H1R3 MYLK2_HUMAN:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

タンパク質配列:
MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKATGLKLAAKVIKKQTPKDKEMVLLEIEVMNQLNHRNLIQLYAAIETPHEIVLFMEYIEGGELFERIVDEDYHLTEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPNEKLKVNFGTPEFLSPEVVNYDQISDKTDMWSMGVITYMLLSGLSPFLGDDDTETLNNVLSGNWYFDEETFEAVSDEAKDFVSNLIVKDQRARMNAAQCLAHPWLNNLAEKAKRCNRRLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H1R3 基板として

Site PTM Type Enzyme
S143 Phosphorylation
S151 Phosphorylation P49137 (MAPKAPK2)
K561 Ubiquitination
Y563 Phosphorylation
K584 Ubiquitination
S586 Phosphorylation
S587 Phosphorylation
S588 Phosphorylation

PTMs - Q9H1R3 酵素として

Substrate Site Source
Q06413 (MEF2C) T80 Uniprot

研究背景

機能:

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.

細胞の位置付け:

Cytoplasm.
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

サブユニット構造:

May interact with centrin.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

参考文献

1). Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats. Cell Transplantation, 2020 (PubMed: 33081508) [IF=3.3]

Application: WB    Species: rat    Sample: muscle

Figure 6. |MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (B) The expression of MLCK protein. MLCK expression was increased in the MPS group compared with that in the control group. MLCK expression was decreased in the ERK inhibitor group compared with that in the MPS group.

Application: WB    Species: Rat    Sample: muscle tissue

Figure 6. MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (A) The relationship between ERK and MLCK was shown by bioinformatics prediction with the STRING database. (B) The expression of MLCK protein. MLCK expression was increased in the MPS group compared with that in the control group. MLCK expression was decreased in the ERK inhibitor group compared with that in the MPS group. Data are presented as the mean ± SEM (n = 6). The data were analyzed using one-way ANOVA. **P < 0.01. (C) The expression of p-MLC protein. p-MLC expression was increased in the MPS group compared with that in the control group. p-MLC expression was decreased in the ERK inhibitor group compared with that in the MPS group. Data are presented as the mean ± SEM (n = 6). The data were analyzed using one-way ANOVA. **P < 0.01. ANOVA: analysis of variance; MLCK: myosin light chain kinase; MPS: myofascial pain syndrome; SEM: standard error of the mean.
2). Dexmedetomidine Inhibited Peripheral Platelet-Derived Growth Factor Receptor Α Regulated Myofascial Trigger Points Pain and Muscle Fibers Contraction Via JAK2/STAT3 Signaling Pathway. , 2022

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