TNIK Antibody - #DF9050
| 製品: | TNIK Antibody |
| カタログ: | DF9050 |
| タンパク質の説明: | Rabbit polyclonal antibody to TNIK |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 155 kDa; 155kD(Calculated). |
| ユニプロット: | Q9UKE5 |
| RRID: | AB_2842246 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9050, RRID:AB_2842246.
折りたたみ／展開
EC 2.7.11.1; KIAA0551; Tnik; TNIK_HUMAN; Traf2 and NCK interacting kinase; Traf2 and NCK-interacting protein kinase;
免疫原
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
- Q9UKE5 TNIK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPILESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPAKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPMKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UKE5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| Y475 | Phosphorylation | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| Y519 | Phosphorylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| T643 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| R698 | Methylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| R705 | Methylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| K824 | Acetylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| T942 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| S959 | Phosphorylation | Uniprot | |
| Y963 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| Y982 | Phosphorylation | Uniprot | |
| T987 | Phosphorylation | Uniprot | |
| S1021 | Phosphorylation | Uniprot | |
| T1036 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | Uniprot | |
| K1131 | Ubiquitination | Uniprot | |
| K1178 | Ubiquitination | Uniprot | |
| Y1264 | Phosphorylation | Uniprot | |
| T1267 | Phosphorylation | Uniprot | |
| K1319 | Ubiquitination | Uniprot | |
| K1325 | Ubiquitination | Uniprot | |
| K1333 | Ubiquitination | Uniprot |
PTMs - Q9UKE5 酵素として
研究背景
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm>Cytoskeleton.
Note: Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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