GEMIN8 Antibody - #DF9077
| 製品: | GEMIN8 Antibody |
| カタログ: | DF9077 |
| タンパク質の説明: | Rabbit polyclonal antibody to GEMIN8 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 29kDa; 29kD(Calculated). |
| ユニプロット: | Q9NWZ8 |
| RRID: | AB_2842273 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9077, RRID:AB_2842273.
折りたたみ／展開
FAM51A1; FLJ20514; FLJ43072; gem (nuclear organelle) associated protein 8;
免疫原
- Q9NWZ8 GEMI8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NWZ8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot |
研究背景
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
Nucleus>Gem. Cytoplasm.
Note: Found in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.
Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with SMN1 and with the GEMIN6-GEMIN7 heterodimer, mediating their interaction.
研究領域
· Genetic Information Processing > Translation > RNA transport.
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