ORC2L Antibody - #DF9085
| 製品: | ORC2L Antibody |
| カタログ: | DF9085 |
| タンパク質の説明: | Rabbit polyclonal antibody to ORC2L |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 66 kDa; 66kD(Calculated). |
| ユニプロット: | Q13416 |
| RRID: | AB_2842281 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9085, RRID:AB_2842281.
折りたたみ／展開
orc2; ORC2_HUMAN; ORC2L; Origin of replication 2 (yeast homolog) like; Origin recognition complex protein 2 homolog; Origin recognition complex subunit 2; Origin recognition complex subunit 2 isoform B; Origin recognition complex subunit 2 like (yeast); Origin recognition complex, subunit 2 homolog; Origin recognition complex, subunit 2, S. cerevisiae, homolog of; Origin recognition complex, subunit 2-like (S. cerevisiae);
免疫原
- Q13416 ORC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13416 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K47 | Acetylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| Y95 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S122 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| K126 | Sumoylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K147 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T202 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S228 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| K549 | Ubiquitination | Uniprot |
研究背景
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
Nucleus.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4 (By similarity). Interacts with MCM10. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein. Interacts with POLQ.
Belongs to the ORC2 family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.