製品: RPS10 Antibody
カタログ: DF9116
タンパク質の説明: Rabbit polyclonal antibody to RPS10
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 19 kDa; 19kD(Calculated).
ユニプロット: P46783
RRID: AB_2842312

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
RPS10 Antibody detects endogenous levels of total RPS10.
RRID:
AB_2842312
引用形式: Affinity Biosciences Cat# DF9116, RRID:AB_2842312.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

40S ribosomal protein S10; DBA9; MGC88819; OTTHUMP00000016229; OTTHUMP00000016230; RPS10; RS10_HUMAN; S10;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
90
Pig
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46783 基板として

Site PTM Type Enzyme
Y12 Phosphorylation
K17 Acetylation
K17 Ubiquitination
K24 Acetylation
K24 Ubiquitination
K31 Ubiquitination
K38 Acetylation
K38 Ubiquitination
K47 Ubiquitination
K53 Ubiquitination
K59 Acetylation
K59 Ubiquitination
Y78 Phosphorylation
Y82 Phosphorylation
T93 Phosphorylation
T101 Phosphorylation
K107 Sumoylation
K107 Ubiquitination
T118 Phosphorylation Q96GD4 (AURKB)
R119 Methylation
T126 Phosphorylation
Y127 Phosphorylation
S130 Phosphorylation
K138 Sumoylation
K138 Ubiquitination
K139 Methylation
K139 Sumoylation
K139 Ubiquitination
S146 Phosphorylation
T148 Phosphorylation
R153 Methylation
R158 Methylation
R160 Methylation

研究背景

機能:

Component of the 40S ribosomal subunit.

PTMs:

Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.

Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.

細胞の位置付け:

Cytoplasm. Nucleus>Nucleolus.
Note: Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Colocalizes with NPS1 in the GC region of the nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the small ribosomal subunit. Interacts with PRMT5. The methylated form interacts with NPM1.

タンパク質ファミリー:

Belongs to the eukaryotic ribosomal protein eS10 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

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