RPL13A Antibody - #DF9126
| 製品: | RPL13A Antibody |
| カタログ: | DF9126 |
| タンパク質の説明: | Rabbit polyclonal antibody to RPL13A |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Zebrafish, Bovine, Horse, Dog, Xenopus |
| 分子量: | 24 kDa; 24kD(Calculated). |
| ユニプロット: | P40429 |
| RRID: | AB_2842322 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9126, RRID:AB_2842322.
折りたたみ／展開
23 kD highly basic protein; 23 kDa highly basic protein; 60S ribosomal protein L13a; L13A; P198; Ribosomal protein L13a; RPL 13A; Tissue specific transplantation antigen 1; Transplantation antigen P198; TSTA1; Tstap198 7; Tum antigen; Tum P198 antigen;
免疫原
- P40429 RL13A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P40429 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| A2 | Acetylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| C38 | S-Nitrosylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| R68 | Methylation | Uniprot | |
| R74 | Methylation | Uniprot | |
| S77 | Phosphorylation | P53355 (DAPK1) , O43293 (DAPK3) , PR:000037784 (hDAPK3/Phos:1) | Uniprot |
| K91 | Ubiquitination | Uniprot | |
| R101 | Methylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| K148 | Acetylation | Uniprot | |
| K148 | Methylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K163 | Acetylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K191 | Acetylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot |
研究背景
Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.
Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.
Citrullinated by PADI4.
Cytoplasm.
Component of the 60S ribosome. Component of the GAIT complex. Interacts with EIF4G1.
Belongs to the universal ribosomal protein uL13 family.
研究領域
· Genetic Information Processing > Translation > Ribosome.
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