製品: RPL27 Antibody
カタログ: DF9129
タンパク質の説明: Rabbit polyclonal antibody to RPL27
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 16 kDa; 16kD(Calculated).
ユニプロット: P61353
RRID: AB_2842325

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
RPL27 Antibody detects endogenous levels of total RPL27.
RRID:
AB_2842325
引用形式: Affinity Biosciences Cat# DF9129, RRID:AB_2842325.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

60S ribosomal protein L27; Ribosomal protein L27; RPL 27;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
92
Zebrafish
92
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61353 基板として

Site PTM Type Enzyme
K6 Ubiquitination
K9 Ubiquitination
K27 Acetylation
K27 Ubiquitination
R36 Methylation
Y49 Phosphorylation
K52 Ubiquitination
K59 Ubiquitination
K73 Ubiquitination
Y75 Phosphorylation
Y77 Phosphorylation
T83 Phosphorylation
R84 Methylation
S86 Phosphorylation
K93 Acetylation
K93 Ubiquitination
K98 Acetylation
K98 Sumoylation
K98 Ubiquitination
K107 Ubiquitination
K115 Ubiquitination
K117 Acetylation
K117 Sumoylation
K117 Ubiquitination
K128 Acetylation
K128 Ubiquitination
K133 Acetylation
K133 Ubiquitination

研究背景

機能:

Component of the large ribosomal subunit. Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs.

細胞の位置付け:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the large ribosomal subunit. Interacts with RRP1B. Interacts with DHX33.

タンパク質ファミリー:

Belongs to the eukaryotic ribosomal protein eL27 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

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