AAAS Antibody - #DF9182
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9182, RRID:AB_2842378.
折りたたみ／展開
AAA; AAAS; AAASb; Achalasia adrenocortical insufficiency alacrimia (Allgrove triple A); Achalasia adrenocortical insufficiency alacrimia; ADRACALA; ADRACALIN; Aladin; Allgrove triple A; DKFZp586G1624; GL003;
免疫原
Widely expressed (PubMed:11159947, PubMed:16022285). Particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures and fetal lung (PubMed:11159947).
- Q9NRG9 AAAS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCSLGLFPPPPPRGQVTLYEHNNELVTGSSYESPPPDFRGQWINLPVLQLTKDPLKTPGRLDHGTRTAFIHHREQVWKRCINIWRDVGLFGVLNEIANSEEEVFEWVKTASGWALALCRWASSLHGSLFPHLSLRSEDLIAEFAQVTNWSSCCLRVFAWHPHTNKFAVALLDDSVRVYNASSTIVPSLKHRLQRNVASLAWKPLSASVLAVACQSCILIWTLDPTSLSTRPSSGCAQVLSHPGHTPVTSLAWAPSGGRLLSASPVDAAIRVWDVSTETCVPLPWFRGGGVTNLLWSPDGSKILATTPSAVFRVWEAQMWTCERWPTLSGRCQTGCWSPDGSRLLFTVLGEPLIYSLSFPERCGEGKGCVGGAKSATIVADLSETTIQTPDGEERLGGEAHSMVWDPSGERLAVLMKGKPRVQDGKPVILLFRTRNSPVFELLPCGIIQGEPGAQPQLITFHPSFNKGALLSVGWSTGRIAHIPLYFVNAQFPRFSPVLGRAQEPPAGGGGSIHDLPLFTETSPTSAPWDPLPGPPPVLPHSPHSHL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NRG9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C2 | Acetylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot |
研究背景
Plays a role in the normal development of the peripheral and central nervous system. Required for the correct localization of aurora kinase AURKA and the microtubule minus end-binding protein NUMA1 as well as a subset of AURKA targets which ensures proper spindle formation and timely chromosome alignment.
Nucleus>Nuclear pore complex. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In metaphase cells localizes within the spindle with some accumulation around spindle poles, with the highest concentration between the centrosome and metaphase plate (PubMed:26246606). The localization to the spindle is microtubule-mediated (PubMed:26246606).
Widely expressed. Particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures and fetal lung.
Interacts with NDC1, the interaction is required for nuclear pore localization. Interacts with the inactive form aurora kinase AURKA.
研究領域
· Genetic Information Processing > Translation > RNA transport.
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