AMOT Antibody - #DF9208
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9208, RRID:AB_2842404.
折りたたみ／展開
AMOT; AMOT_HUMAN; Angiomotin; Angiomotin p130 isoform; KIAA1071;
免疫原
Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
- Q4VCS5 AMOT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRNSEEQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNGPPFPSGSGNPGPQSDVLSPQDHHQQLVAHAARQEPQGQEIQSENLIMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQKMRTEGRPSVQRLNPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYKNPTSSSEFYKAQGPLPNQHSLKGMEHRGPPPEYPFKGMPPQSVVCKPQEPGHFYSEHRLNQPGRTEGQLMRYQHPPEYGAARPAQDISLPLSARNSQPHSPTSSLTSGGSLPLLQSPPSTRLSPARHPLVPNQGDHSAHLPRPQQHFLPNQAHQGDHYRLSQPGLSQQQQQQQQQHHHHHHHQQQQQQQPQQQPGEAYSAMPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q4VCS5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R24 | Methylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Q9NRM7 (LATS2) | Uniprot |
| K219 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| R236 | Methylation | Uniprot | |
| R280 | Methylation | Uniprot | |
| R291 | Methylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| R335 | Methylation | Uniprot | |
| R412 | Methylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| T536 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| K545 | Acetylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| R575 | Methylation | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K594 | Sumoylation | Uniprot | |
| K595 | Sumoylation | Uniprot | |
| Y599 | Phosphorylation | Uniprot | |
| K680 | Ubiquitination | Uniprot | |
| S712 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| T717 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| Y719 | Phosphorylation | Uniprot | |
| K739 | Ubiquitination | Uniprot | |
| T750 | Phosphorylation | Uniprot | |
| K758 | Ubiquitination | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| Y836 | Phosphorylation | Uniprot | |
| S842 | Phosphorylation | Uniprot | |
| S847 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T848 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S852 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S855 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1059 | Phosphorylation | Uniprot | |
| T1061 | Phosphorylation | Uniprot |
研究背景
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.
Cell junction>Tight junction.
Note: Localized on the cell surface. May act as a transmembrane protein.
Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.
The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin.
Belongs to the angiomotin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
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