ATXN7 Antibody - #DF9233
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9233, RRID:AB_2842429.
折りたたみ／展開
ADCAII; ATXN 7; OPCA III; OPCA3; SCA 7; SCA7; Spinocerebellar Ataxia 7; Spinocerebellar ataxia type 7 protein;
免疫原
Isoform a and isoform b are expressed in CNS, but isoform a is expressed predominantly in the peripherical tissues. Isoform b is also highly expressed in the frontal lobe, skeletal muscle and spinal cord and is expressed at a lower level in the lung, lymphoblast and intestine.
- O15265 ATX7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSERAADDVRGEPRRAAAAAGGAAAAAARQQQQQQQQQQPPPPQPQRQQHPPPPPRRTRPEDGGPGAASTSAAAMATVGERRPLPSPEVMLGQSWNLWVEASKLPGKDGTELDESFKEFGKNREVMGLCREDMPIFGFCPAHDDFYLVVCNDCNQVVKPQAFQSHYERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15265 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S69 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K257 | Acetylation | Uniprot | |
| K257 | Sumoylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| R531 | Methylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S651 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| S845 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| T854 | Phosphorylation | Uniprot |
研究背景
Acts as component of the STAGA transcription coactivator-HAT complex. Mediates the interaction of STAGA complex with the CRX and is involved in CRX-dependent gene activation. Necessary for microtubule cytoskeleton stabilization.
Proteolytically cleaved. The cleavage may be involved in SCA7 degeneration: the isoform fragments may exert distinct toxic influences that could contribute to selective neurodegeneration.
Sumoylation decreases the aggregation propensity and cellular toxicity of forms with an expanded poly-Gln region but has no effect on subcellular location or interaction with components of the STAGA complex.
Nucleus. Nucleus>Nucleolus. Nucleus matrix. Cytoplasm>Cytoskeleton.
Note: In addition to a diffuse distribution throughout the nucleus, it is associated with the nuclear matrix and the nucleolus. It is able to shuttle between the nucleus and cytoplasm.
Cytoplasm.
Isoform a and isoform b are expressed in CNS, but isoform a is expressed predominantly in the peripherical tissues. Isoform b is also highly expressed in the frontal lobe, skeletal muscle and spinal cord and is expressed at a lower level in the lung, lymphoblast and intestine.
Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and TAF10. Interacts with alpha tubulin.
Belongs to the ataxin-7 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.