DDX42 Antibody - #DF9259
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9259, RRID:AB_2842455.
折りたたみ／展開
ATP-dependent RNA helicase DDX42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box polypeptide 42 protein; DEAD box protein 42; EC 3.6.1.-; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD-box protein; SF3b125; SF3b125 DEAD-box protein; Splicing factor 3B-associated 125 kDa protein;
免疫原
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
- Q86XP3 DDX42_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86XP3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Acetylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K50 | Methylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K145 | Acetylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K270 | Sumoylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| K626 | Acetylation | Uniprot | |
| R673 | Methylation | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| K686 | Acetylation | Uniprot | |
| K700 | Methylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| Y707 | Phosphorylation | Uniprot | |
| K708 | Methylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| K806 | Acetylation | Uniprot | |
| R814 | Methylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| Y844 | Phosphorylation | Uniprot | |
| R875 | Methylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| K899 | Sumoylation | Uniprot | |
| S914 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot |
研究背景
ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
Cytoplasm. Nucleus speckle. Nucleus>Cajal body.
Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts (via the C-terminus) with TP53BP2; the interaction is not inhibitied by TP53BP2 ubiquitination and is independent of p53/TP53.
Belongs to the DEAD box helicase family. DDX42 subfamily.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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