製品: BRAP Antibody
カタログ: DF9289
タンパク質の説明: Rabbit polyclonal antibody to BRAP
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 67 kDa; 67kD(Calculated).
ユニプロット: Q7Z569
RRID: AB_2842485

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 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(89%), Sheep(100%), Dog(100%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
BRAP Antibody detects endogenous levels of total BRAP.
RRID:
AB_2842485
引用形式: Affinity Biosciences Cat# DF9289, RRID:AB_2842485.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

3010002G07Rik; BRAP2; BRCA1 associated protein; EC 6.3.2.; Galectin 2 binding protein; IMP; Impedes mitogenic signal propagation; Renal carcinoma antigen NY REN 63; RING finger protein 52; RNF52; zgc:92894;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q7Z569 BRAP_HUMAN:

Expressed in breast epithelial cell lines.

タンパク質配列:
MSVSLVVIRLELAEHSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAIIHQHLGRREMTDVIIETMKSNPDELKTTVEERKSSEASPTAQRSKDHSKECINAAPDSPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCILTVPAAMTSHDLMKFVAPFNEVIEQMKIIRDSTPNQYMVLIKFRAQADADSFYMTCNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLKEKQSVERKCTQLNTKVAKLTNELKEEQEMNKCLRANQVLLQNKLKEEERVLKETCDQKDLQITEIQEQLRDVMFYLETQQKINHLPAETRQEIQEGQINIAMASASSPASSGGSGKLPSRKGRSKRGK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
89
Xenopus
89
Zebrafish
75
Chicken
63
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z569 基板として

Site PTM Type Enzyme
K38 Ubiquitination
S43 Phosphorylation
K51 Ubiquitination
S52 Phosphorylation
S79 Phosphorylation
K85 Acetylation
K85 Ubiquitination
S93 Phosphorylation
S94 Phosphorylation
S97 Phosphorylation
T99 Phosphorylation
S107 Phosphorylation
S117 Phosphorylation
S119 Phosphorylation
K154 Ubiquitination
T173 Phosphorylation
S286 Phosphorylation
S289 Phosphorylation
T308 Phosphorylation
T352 Phosphorylation
T361 Phosphorylation
K381 Ubiquitination
K385 Ubiquitination
Y389 Phosphorylation
K400 Ubiquitination
Y422 Phosphorylation
K426 Ubiquitination
K444 Ubiquitination
K446 Ubiquitination
K458 Acetylation
K458 Ubiquitination
K464 Ubiquitination
K482 Ubiquitination
K507 Ubiquitination
K522 Ubiquitination
S570 Phosphorylation
K580 Ubiquitination

研究背景

機能:

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in breast epithelial cell lines.

サブユニット構造:

Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.

研究領域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

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