PDE5A Antibody - #DF9373
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| 製品: | PDE5A Antibody |
| カタログ: | DF9373 |
| タンパク質の説明: | Rabbit polyclonal antibody to PDE5A |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 100 kDa; 100kD(Calculated). |
| ユニプロット: | O76074 |
| RRID: | AB_2842569 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9373, RRID:AB_2842569.
折りたたみ／展開
5''-cyclic phosphodiesterase; CGB PDE; CGB-PDE; CGBPDE; cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific phosphodiesterase; cGMP binding/cGMP specific phosphodiesterase; cGMP specific 3'5' cyclic phosphodiesterase; cGMP specific phosphodiesterase PDE5A2; cGMP specific phosphodiesterase type 5A; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3''; CN5A; CN5N; PDE 5; PDE 5A; PDE5; Pde5a; PDE5A_HUMAN; PDE5A1; Phosphodiesterase 5A; Phosphodiesterase 5A cGMP specific; Phosphodiesterase isozyme 5;
免疫原
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
- O76074 PDE5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O76074 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S86 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) | Uniprot |
| S104 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| K714 | Ubiquitination | Uniprot | |
| S869 | Phosphorylation | Uniprot |
研究背景
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation.
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
Belongs to the cyclic nucleotide phosphodiesterase family.
研究領域
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Metabolism > Nucleotide metabolism > Purine metabolism.
参考文献
Application: IHC Species: rat Sample: flap
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