CLCN2 Antibody - #DF9375
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9375, RRID:AB_2842571.
折りたたみ／展開
Chloride Channel 2; Chloride channel protein 2; Chloride channel, voltage sensitive 2; CIC 2; CIC2; ClC-2; CLC2; Clcn2; CLCN2_HUMAN; ECA2; ECA3; EG13; EGI11; EGMA; EJM6; EJM8; PKA-activated chloride channel;
免疫原
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. Expressed in the adrenal gland, predominantly in the zona glomerulosa (PubMed:29403011).
- P51788 CLCN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAAEEGMEPRALQYEQTLMYGRYTQDLGAFAKEEAARIRLGGPEPWKGPPSSRAAPELLEYGRSRCARCRVCSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWVMDYAIAACLQAQQWMSRGLNTSILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFIAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEELEPPSTSQAWNPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51788 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T607 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| K693 | Acetylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S712 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| T801 | Phosphorylation | Uniprot | |
| S802 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot |
研究背景
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Involved in the regulation of aldosterone production. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated Ca2+ channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis.
Phosphorylated. Activated by dephosphorylation.
Cell membrane>Multi-pass membrane protein.
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. Expressed in the adrenal gland, predominantly in the zona glomerulosa.
Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily.
研究領域
· Organismal Systems > Digestive system > Mineral absorption.
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