製品: F13B Antibody
カタログ: DF9401
タンパク質の説明: Rabbit polyclonal antibody to F13B
アプリケーション: WB IHC IF/ICC
反応性: Human, Monkey
予測: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
分子量: 76kDa; 76kD(Calculated).
ユニプロット: P05160
RRID: AB_2842597

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Monkey
予測:
Pig(100%), Zebrafish(83%), Bovine(86%), Sheep(100%), Rabbit(86%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
F13B Antibody detects endogenous levels of total F13B.
RRID:
AB_2842597
引用形式: Affinity Biosciences Cat# DF9401, RRID:AB_2842597.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Coagulation factor XIII, A2 polypeptide; Coagulation factor XIII, B polypeptide; F13A2; F13B; FXIIIB;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MRLKNLTFIIILIISGELYAEEKPCGFPHVENGRIAQYYYTFKSFYFPMSIDKKLSFFCLAGYTTESGRQEEQTTCTTEGWSPEPRCFKKCTKPDLSNGYISDVKLLYKIQENMRYGCASGYKTTGGKDEEVVQCLSDGWSSQPTCRKEHETCLAPELYNGNYSTTQKTFKVKDKVQYECATGYYTAGGKKTEEVECLTYGWSLTPKCTKLKCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPVCEGRRNRCPPPPLPINSKIQTHSTTYRHGEIVHIECELNFEIHGSAEIRCEDGKWTEPPKCIEGQEKVACEEPPFIENGAANLHSKIYYNGDKVTYACKSGYLLHGSNEITCNRGKWTLPPECVENNENCKHPPVVMNGAVADGILASYATGSSVEYRCNEYYLLRGSKISRCEQGKWSSPPVCLEPCTVNVDYMNRNNIEMKWKYEGKVLHGDLIDFVCKQGYDLSPLTPLSELSVQCNRGEVKYPLCTRKESKGMCTSPPLIKHGVIISSTVDTYENGSSVEYRCFDHHFLEGSREAYCLDGMWTTPPLCLEPCTLSFTEMEKNNLLLKWDFDNRPHILHGEYIEFICRGDTYPAELYITGSILRMQCDRGQLKYPRCIPRQSTLSYQEPLRT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Bovine
86
Rabbit
86
Zebrafish
83
Horse
71
Chicken
50
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05160 基板として

Site PTM Type Enzyme
N162 N-Glycosylation
T182 Phosphorylation
S373 Phosphorylation
Y512 Phosphorylation
N545 N-Glycosylation
Y643 Phosphorylation
T661 Phosphorylation

研究背景

機能:

The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Tetramer of two A chains (F13A1) and two B (F13B) chains.

研究領域

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

参考文献

1). Cationic Zinc is Required for Factor XII Recruitment and Activation by Stimulated Platelets and for Thrombus Formation in vivo. JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2020 (PubMed: 32542960) [IF=10.4]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 5 Factor XII deficiency inhibits thrombus formation in vivo. A, Wild-type (WT) or FXII knockout mice (FXII KO) mice underwent quantitative assessment of thrombus formation in the cremaster arteriole laser injury model (platelet accumulation in red, fibrin formation in green). Representative intravital images are shown, together with median curves and area under the curve (AUC) distributions for platelet accumulation (B-C) and fibrin formation (D-E).

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