Phospho-eIF4E (Ser209) Antibody - #AF3110
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3110, RRID:AB_2834547.
折りたたみ／展開
AUTS19; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; mRNA cap binding protein; mRNA cap-binding protein;
免疫原
- P06730 IF4E_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P06730 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K119 | Acetylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| R173 | Methylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Q9HBH9 (MKNK2) , Q9BUB5 (MKNK1) , O00443 (PIK3C2A) | Uniprot |
| T210 | Phosphorylation | O00443 (PIK3C2A) | Uniprot |
| T211 | Phosphorylation | Uniprot |
研究背景
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Cytoplasm>P-body. Cytoplasm.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with CLOCK (By similarity). Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1. Interacts with METTL3. Interacts with RBM24; this interaction prevents EIF4E from binding to p53/TP53 mRNA and inhibits the assembly of translation initiation complex.
(Microbial infection) Interacts with Lassa virus Z protein.
Belongs to the eukaryotic initiation factor 4E family.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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