POLR1D Antibody - #DF9464

(1)   (16)  
製品: POLR1D Antibody
カタログ: DF9464
タンパク質の説明: Rabbit polyclonal antibody to POLR1D
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 15 kDa; 14kD,15kD(Calculated).
ユニプロット: Q9Y2S0 | P0DPB5 | P0DPB6
RRID: AB_2842660

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MSDS
説明書
COA
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
POLR1D Antibody detects endogenous levels of total POLR1D.
RRID:
AB_2842660
引用形式: Affinity Biosciences Cat# DF9464, RRID:AB_2842660.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AC19; DNA directed RNA polymerase I 16 kDa polypeptide; DNA directed RNA polymerase I subunit D; DNA directed RNA polymerases I and III subunit RPAC2; DNA-directed RNA polymerase I subunit D; DNA-directed RNA polymerases I and III subunit RPAC2; FLJ20616; hRPA19; MGC9850; POLR1C; POLR1D; Polymerase (RNA) I polypeptide D; Polymerase (RNA) I polypeptide D, 16kDa; RNA polymerase I 16 kDa subunit; RNA polymerases I and III subunit AC2; RPA16; RPA9; RPAC2; RPAC2_HUMAN; RPC16; RPO1 3;

免疫原

免疫原:

A synthesized peptide derived from human POLR1D, corresponding to a region within C-terminal amino acids.

Uniprot:

P0DPB5(RPC22_HUMAN) >>Visit HPA database.

P0DPB6(RPAC2_HUMAN) >>Visit HPA database.

タンパク質配列:
MEEDQELERKAIEELLKEAKRGKTRAETMGPMGWMKCPLASTNKRFLINTIKNTLPSHKEQDHEQKEGDKEPAKSQAQKEENPKKHRSHPYKHSFRARGSASYSPPRKRSSQDKYEKRSNRR

MEEDQELERKISGLKTSMAEGERKTALEMVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRGTLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYKDQKASRNESTF

研究背景

機能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively.

タンパク質ファミリー:

Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

研究領域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

参考文献

1). POLR1D promotes colorectal cancer progression and predicts poor prognosis of patients. MOLECULAR CARCINOGENESIS, 2019 (PubMed: 30582221) [IF=4.6]

Application: WB    Species: human    Sample: HCT116 and LOVO cells

Fig.3 |POLR1D knockdown inhibited proliferation and migration of CRC cells. (A) HCT116 and LOVO cells were infected with lentiviral POLR1D shRNA (KD) or a negative control shRNA (NC).The mRNA level of POLR1D was measured by reverse-transcription PCR. (B) POLR1D protein level in HCT116 and LOVO cells infected with lentiviral POLR1D shRNA (KD) or a negative control shRNA (NC)were confirmed by Western blotting.

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