DCTN4 Antibody - #DF9468
| 製品: | DCTN4 Antibody |
| カタログ: | DF9468 |
| タンパク質の説明: | Rabbit polyclonal antibody to DCTN4 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 52 kDa; 52kD(Calculated). |
| ユニプロット: | Q9UJW0 |
| RRID: | AB_2842664 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9468, RRID:AB_2842664.
折りたたみ／展開
DCTN 4; Dctn4; DCTN4_HUMAN; Dynactin 4 (p62); Dynactin 4; Dynactin p62 subunit; Dynactin subunit 4; Dynactin subunit p62; Dynactin4; p62;
免疫原
- Q9UJW0 DCTN4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASLLQSDRVLYLVQGEKKVRAPLSQLYFCRYCSELRSLECVSHEVDSHYCPSCLENMPSAEAKLKKNRCANCFDCPGCMHTLSTRATSISTQLPDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPHTQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSDKYGLGTRLQRPRAGASISTLAGLSLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKRSLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFECEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEGEVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UJW0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R9 | Methylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| Y376 | Phosphorylation | Uniprot |
研究背景
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Stress fiber. Cytoplasm>Cell cortex. Cytoplasm>Myofibril>Sarcomere.
Note: Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity).
Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres (By similarity).
Belongs to the dynactin subunit 4 family.
研究領域
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
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