DYNC1H1 Antibody - #DF9469

(5)  
製品: DYNC1H1 Antibody
カタログ: DF9469
タンパク質の説明: Rabbit polyclonal antibody to DYNC1H1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit, Dog, Chicken
分子量: 532kDa; 532kD(Calculated).
ユニプロット: Q14204
RRID: AB_2842665

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
DYNC1H1 Antibody detects endogenous levels of total DYNC1H1.
RRID:
AB_2842665
引用形式: Affinity Biosciences Cat# DF9469, RRID:AB_2842665.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

cytoplasmic dynein 1 heavy chain 1; cytoplasmic dynein heavy chain 1; DHC1; DHC1a; DKFZp686P2245; DNCH1; Dnchc1; DNECL; DYHC; dynein heavy chain, cytosolic; dynein, cytoplasmic 1, heavy chain 1; dynein, cytoplasmic-like; HL-3; KIAA0325; p22;

免疫原

免疫原:
Uniprot:

Q14204(DYHC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
DYNC1H1(1778)
タンパク質配列:
MSEPGGGGGEDGSAGLEVSAVQNVADVSVLQKHLRKLVPLLLEDGGEAPAALEAALEEKSALEQMRKFLSDPQVHTVLVERSTLKEDVGDEGEEEKEFISYNINIDIHYGVKSNSLAFIKRTPVIDADKPVSSQLRVLTLSEDSPYETLHSFISNAVAPFFKSYIRESGKADRDGDKMAPSVEKKIAELEMGLLHLQQNIEIPEISLPIHPMITNVAKQCYERGEKPKVTDFGDKVEDPTFLNQLQSGVNRWIREIQKVTKLDRDPASGTALQEISFWLNLERALYRIQEKRESPEVLLTLDILKHGKRFHATVSFDTDTGLKQALETVNDYNPLMKDFPLNDLLSATELDKIRQALVAIFTHLRKIRNTKYPIQRALRLVEAISRDLSSQLLKVLGTRKLMHVAYEEFEKVMVACFEVFQTWDDEYEKLQVLLRDIVKRKREENLKMVWRINPAHRKLQARLDQMRKFRRQHEQLRAVIVRVLRPQVTAVAQQNQGEVPEPQDMKVAEVLFDAADANAIEEVNLAYENVKEVDGLDVSKEGTEAWEAAMKRYDERIDRVETRITARLRDQLGTAKNANEMFRIFSRFNALFVRPHIRGAIREYQTQLIQRVKDDIESLHDKFKVQYPQSQACKMSHVRDLPPVSGSIIWAKQIDRQLTAYMKRVEDVLGKGWENHVEGQKLKQDGDSFRMKLNTQEIFDDWARKVQQRNLGVSGRIFTIESTRVRGRTGNVLKLKVNFLPEIITLSKEVRNLKWLGFRVPLAIVNKAHQANQLYPFAISLIESVRTYERTCEKVEERNTISLLVAGLKKEVQALIAEGIALVWESYKLDPYVQRLAETVFNFQEKVDDLLIIEEKIDLEVRSLETCMYDHKTFSEILNRVQKAVDDLNLHSYSNLPIWVNKLDMEIERILGVRLQAGLRAWTQVLLGQAEDKAEVDMDTDAPQVSHKPGGEPKIKNVVHELRITNQVIYLNPPIEECRYKLYQEMFAWKMVVLSLPRIQSQRYQVGVHYELTEEEKFYRNALTRMPDGPVALEESYSAVMGIVSEVEQYVKVWLQYQCLWDMQAENIYNRLGEDLNKWQALLVQIRKARGTFDNAETKKEFGPVVIDYGKVQSKVNLKYDSWHKEVLSKFGQMLGSNMTEFHSQISKSRQELEQHSVDTASTSDAVTFITYVQSLKRKIKQFEKQVELYRNGQRLLEKQRFQFPPSWLYIDNIEGEWGAFNDIMRRKDSAIQQQVANLQMKIVQEDRAVESRTTDLLTDWEKTKPVTGNLRPEEALQALTIYEGKFGRLKDDREKCAKAKEALELTDTGLLSGSEERVQVALEELQDLKGVWSELSKVWEQIDQMKEQPWVSVQPRKLRQNLDALLNQLKSFPARLRQYASYEFVQRLLKGYMKINMLVIELKSEALKDRHWKQLMKRLHVNWVVSELTLGQIWDVDLQKNEAIVKDVLLVAQGEMALEEFLKQIREVWNTYELDLVNYQNKCRLIRGWDDLFNKVKEHINSVSAMKLSPYYKVFEEDALSWEDKLNRIMALFDVWIDVQRRWVYLEGIFTGSADIKHLLPVETQRFQSISTEFLALMKKVSKSPLVMDVLNIQGVQRSLERLADLLGKIQKALGEYLERERSSFPRFYFVGDEDLLEIIGNSKNVAKLQKHFKKMFAGVSSIILNEDNSVVLGISSREGEEVMFKTPVSITEHPKINEWLTLVEKEMRVTLAKLLAESVTEVEIFGKATSIDPNTYITWIDKYQAQLVVLSAQIAWSENVETALSSMGGGGDAAPLHSVLSNVEVTLNVLADSVLMEQPPLRRRKLEHLITELVHQRDVTRSLIKSKIDNAKSFEWLSQMRFYFDPKQTDVLQQLSIQMANAKFNYGFEYLGVQDKLVQTPLTDRCYLTMTQALEARLGGSPFGPAGTGKTESVKALGHQLGRFVLVFNCDETFDFQAMGRIFVGLCQVGAWGCFDEFNRLEERMLSAVSQQVQCIQEALREHSNPNYDKTSAPITCELLNKQVKVSPDMAIFITMNPGYAGRSNLPDNLKKLFRSLAMTKPDRQLIAQVMLYSQGFRTAEVLANKIVPFFKLCDEQLSSQSHYDFGLRALKSVLVSAGNVKRERIQKIKREKEERGEAVDEGEIAENLPEQEILIQSVCETMVPKLVAEDIPLLFSLLSDVFPGVQYHRGEMTALREELKKVCQEMYLTYGDGEEVGGMWVEKVLQLYQITQINHGLMMVGPSGSGKSMAWRVLLKALERLEGVEGVAHIIDPKAISKDHLYGTLDPNTREWTDGLFTHVLRKIIDSVRGELQKRQWIVFDGDVDPEWVENLNSVLDDNKLLTLPNGERLSLPPNVRIMFEVQDLKYATLATVSRCGMVWFSEDVLSTDMIFNNFLARLRSIPLDEGEDEAQRRRKGKEDEGEEAASPMLQIQRDAATIMQPYFTSNGLVTKALEHAFQLEHIMDLTRLRCLGSLFSMLHQACRNVAQYNANHPDFPMQIEQLERYIQRYLVYAILWSLSGDSRLKMRAELGEYIRRITTVPLPTAPNIPIIDYEVSISGEWSPWQAKVPQIEVETHKVAAPDVVVPTLDTVRHEALLYTWLAEHKPLVLCGPPGSGKTMTLFSALRALPDMEVVGLNFSSATTPELLLKTFDHYCEYRRTPNGVVLAPVQLGKWLVLFCDEINLPDMDKYGTQRVISFIRQMVEHGGFYRTSDQTWVKLERIQFVGACNPPTDPGRKPLSHRFLRHVPVVYVDYPGPASLTQIYGTFNRAMLRLIPSLRTYAEPLTAAMVEFYTMSQERFTQDTQPHYIYSPREMTRWVRGIFEALRPLETLPVEGLIRIWAHEALRLFQDRLVEDEERRWTDENIDTVALKHFPNIDREKAMSRPILYSNWLSKDYIPVDQEELRDYVKARLKVFYEEELDVPLVLFNEVLDHVLRIDRIFRQPQGHLLLIGVSGAGKTTLSRFVAWMNGLSVYQIKVHRKYTGEDFDEDLRTVLRRSGCKNEKIAFIMDESNVLDSGFLERMNTLLANGEVPGLFEGDEYATLMTQCKEGAQKEGLMLDSHEELYKWFTSQVIRNLHVVFTMNPSSEGLKDRAATSPALFNRCVLNWFGDWSTEALYQVGKEFTSKMDLEKPNYIVPDYMPVVYDKLPQPPSHREAIVNSCVFVHQTLHQANARLAKRGGRTMAITPRHYLDFINHYANLFHEKRSELEEQQMHLNVGLRKIKETVDQVEELRRDLRIKSQELEVKNAAANDKLKKMVKDQQEAEKKKVMSQEIQEQLHKQQEVIADKQMSVKEDLDKVEPAVIEAQNAVKSIKKQHLVEVRSMANPPAAVKLALESICLLLGESTTDWKQIRSIIMRENFIPTIVNFSAEEISDAIREKMKKNYMSNPSYNYEIVNRASLACGPMVKWAIAQLNYADMLKRVEPLRNELQKLEDDAKDNQQKANEVEQMIRDLEASIARYKEEYAVLISEAQAIKADLAAVEAKVNRSTALLKSLSAERERWEKTSETFKNQMSTIAGDCLLSAAFIAYAGYFDQQMRQNLFTTWSHHLQQANIQFRTDIARTEYLSNADERLRWQASSLPADDLCTENAIMLKRFNRYPLIIDPSGQATEFIMNEYKDRKITRTSFLDDAFRKNLESALRFGNPLLVQDVESYDPVLNPVLNREVRRTGGRVLITLGDQDIDLSPSFVIFLSTRDPTVEFPPDLCSRVTFVNFTVTRSSLQSQCLNEVLKAERPDVDEKRSDLLKLQGEFQLRLRQLEKSLLQALNEVKGRILDDDTIITTLENLKREAAEVTRKVEETDIVMQEVETVSQQYLPLSTACSSIYFTMESLKQIHFLYQYSLQFFLDIYHNVLYENPNLKGVTDHTQRLSIITKDLFQVAFNRVARGMLHQDHITFAMLLARIKLKGTVGEPTYDAEFQHFLRGNEIVLSAGSTPRIQGLTVEQAEAVVRLSCLPAFKDLIAKVQADEQFGIWLDSSSPEQTVPYLWSEETPATPIGQAIHRLLLIQAFRPDRLLAMAHMFVSTNLGESFMSIMEQPLDLTHIVGTEVKPNTPVLMCSVPGYDASGHVEDLAAEQNTQITSIAIGSAEGFNQADKAINTAVKSGRWVMLKNVHLAPGWLMQLEKKLHSLQPHACFRLFLTMEINPKVPVNLLRAGRIFVFEPPPGVKANMLRTFSSIPVSRICKSPNERARLYFLLAWFHAIIQERLRYAPLGWSKKYEFGESDLRSACDTVDTWLDDTAKGRQNISPDKIPWSALKTLMAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14204 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S13 Phosphorylation
K36 Ubiquitination
K59 Ubiquitination
K67 Ubiquitination
S70 Phosphorylation
K85 Ubiquitination
K120 Ubiquitination
K129 Ubiquitination
K235 Ubiquitination
T240 Phosphorylation
T318 Phosphorylation
K337 Ubiquitination
S346 Phosphorylation
T348 Phosphorylation
T362 Phosphorylation
K371 Acetylation
K394 Acetylation
K394 Ubiquitination
K400 Ubiquitination
Y406 Phosphorylation
R477 Methylation
S539 Phosphorylation
K540 Ubiquitination
K551 Ubiquitination
T574 Phosphorylation
K576 Ubiquitination
K613 Ubiquitination
K622 Ubiquitination
K624 Ubiquitination
C633 S-Nitrosylation
K634 Ubiquitination
K652 Ubiquitination
K671 Ubiquitination
K681 Ubiquitination
K683 Ubiquitination
S688 Phosphorylation
K692 Acetylation
K692 Ubiquitination
T723 Phosphorylation
K734 Ubiquitination
K736 Ubiquitination
S747 Phosphorylation
K748 Ubiquitination
K754 Acetylation
K754 Ubiquitination
K794 Acetylation
K809 Ubiquitination
S863 Phosphorylation
T866 Phosphorylation
Y869 Phosphorylation
K872 Ubiquitination
T873 Phosphorylation
R880 Methylation
K883 Acetylation
K956 Ubiquitination
Y970 Phosphorylation
K981 Ubiquitination
S995 Phosphorylation
Y1010 Phosphorylation
K1078 Ubiquitination
K1099 Ubiquitination
K1100 Ubiquitination
K1111 Ubiquitination
K1115 Ubiquitination
K1119 Ubiquitination
K1125 Acetylation
K1125 Ubiquitination
K1130 Ubiquitination
S1137 Phosphorylation
S1157 Phosphorylation
S1164 Phosphorylation
T1168 Phosphorylation
Y1172 Phosphorylation
S1175 Phosphorylation
K1185 Acetylation
K1185 Ubiquitination
K1228 Ubiquitination
S1230 Phosphorylation
K1242 Ubiquitination
R1248 Methylation
T1259 Phosphorylation
Y1283 Phosphorylation
K1286 Acetylation
K1286 Ubiquitination
K1301 Ubiquitination
T1307 Phosphorylation
S1313 Phosphorylation
K1330 Ubiquitination
K1347 Ubiquitination
K1371 Acetylation
K1371 Methylation
K1371 Ubiquitination
R1378 Methylation
K1414 Ubiquitination
K1483 Ubiquitination
K1496 Ubiquitination
K1498 Ubiquitination
K1508 Ubiquitination
K1584 Ubiquitination
Y1618 Phosphorylation
S1624 Phosphorylation
K1649 Ubiquitination
K1697 Ubiquitination
Y1738 Phosphorylation
K1807 Ubiquitination
T1822 Phosphorylation
S1824 Phosphorylation
K1834 Ubiquitination
S1835 Phosphorylation
S1840 Phosphorylation
T1851 Phosphorylation
K1878 Ubiquitination
T1882 Phosphorylation
R1887 Methylation
S1903 Phosphorylation
K1912 Ubiquitination
K1917 Ubiquitination
K1992 Ubiquitination
S1994 Phosphorylation
C1999 S-Nitrosylation
K2004 Ubiquitination
K2033 Ubiquitination
T2042 Phosphorylation
Y2055 Phosphorylation
K2068 Ubiquitination
K2094 Ubiquitination
S2095 Phosphorylation
S2099 Phosphorylation
K2104 Ubiquitination
K2239 Ubiquitination
K2257 Ubiquitination
K2261 Ubiquitination
Y2265 Phosphorylation
T2267 Phosphorylation
T2272 Phosphorylation
R2273 Methylation
T2276 Phosphorylation
T2281 Phosphorylation
K2349 Ubiquitination
S2384 Phosphorylation
K2401 Ubiquitination
S2410 Phosphorylation
S2429 Phosphorylation
S2503 Phosphorylation
Y2517 Phosphorylation
S2546 Phosphorylation
T2559 Phosphorylation
K2561 Ubiquitination
T2695 Phosphorylation
K2702 Ubiquitination
K2721 Ubiquitination
Y2735 Phosphorylation
S2743 Phosphorylation
T2745 Phosphorylation
T2750 Phosphorylation
S2761 Phosphorylation
T2770 Phosphorylation
Y2777 Phosphorylation
S2795 Phosphorylation
R2836 Methylation
R2843 Methylation
K2856 Ubiquitination
K2865 Ubiquitination
K2879 Ubiquitination
Y2881 Phosphorylation
Y2892 Phosphorylation
K2966 Ubiquitination
S2997 Phosphorylation
K3039 Ubiquitination
Y3051 Phosphorylation
K3076 Ubiquitination
S3082 Phosphorylation
Y3103 Phosphorylation
K3112 Ubiquitination
K3117 Ubiquitination
Y3125 Phosphorylation
Y3130 Phosphorylation
K3207 Sumoylation
K3209 Ubiquitination
R3219 Methylation
K3225 Ubiquitination
K3253 Ubiquitination
S3257 Phosphorylation
K3284 Ubiquitination
K3301 Ubiquitination
S3309 Phosphorylation
S3323 Phosphorylation
K3369 Ubiquitination
Y3377 Phosphorylation
Y3379 Phosphorylation
S3386 Phosphorylation
K3407 Ubiquitination
K3418 Ubiquitination
K3429 Ubiquitination
S3443 Phosphorylation
Y3447 Phosphorylation
Y3451 Phosphorylation
K3462 Ubiquitination
K3471 Ubiquitination
S3475 Phosphorylation
T3476 Phosphorylation
K3480 Acetylation
K3480 Ubiquitination
K3491 Ubiquitination
Y3552 Phosphorylation
S3554 Phosphorylation
K3581 Ubiquitination
K3605 Ubiquitination
K3621 Ubiquitination
S3640 Phosphorylation
S3706 Phosphorylation
K3718 Ubiquitination
S3729 Phosphorylation
K3747 Ubiquitination
S3748 Phosphorylation
K3757 Ubiquitination
K3774 Acetylation
K3774 Ubiquitination
K3893 Ubiquitination
S3917 Phosphorylation
T3921 Phosphorylation
K3945 Ubiquitination
K4089 Ubiquitination
K4111 Ubiquitination
T4127 Phosphorylation
K4154 Ubiquitination
S4162 Phosphorylation
K4171 Ubiquitination
K4204 Ubiquitination
K4228 Ubiquitination
S4234 Phosphorylation
K4237 Ubiquitination
S4241 Phosphorylation
T4274 Phosphorylation
S4277 Phosphorylation
S4280 Phosphorylation
K4283 Acetylation
K4283 Ubiquitination
K4287 Ubiquitination
K4292 Ubiquitination
S4341 Phosphorylation
K4342 Ubiquitination
K4345 Ubiquitination
K4362 Ubiquitination
T4364 Phosphorylation
T4366 Phosphorylation
S4368 Phosphorylation
T4369 Phosphorylation
S4370 Phosphorylation
K4406 Ubiquitination
K4441 Ubiquitination
K4457 Ubiquitination
K4487 Ubiquitination
S4493 Phosphorylation
K4502 Ubiquitination
K4505 Ubiquitination
S4582 Phosphorylation
T4583 Phosphorylation
T4588 Phosphorylation
K4594 Ubiquitination
S4603 Phosphorylation
Y4610 Phosphorylation
T4614 Phosphorylation

研究背景

機能:

Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression.

細胞の位置付け:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer. Interacts with DYNC1LI1; DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1. Interacts with DYNC1LI2; DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1. Interacts with DYNC1I2 (By similarity). Interacts with BICD2.

タンパク質ファミリー:

Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.

Belongs to the dynein heavy chain family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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