FANCL Antibody - #DF9476
| 製品: | FANCL Antibody |
| カタログ: | DF9476 |
| タンパク質の説明: | Rabbit polyclonal antibody to FANCL |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 43 kDa; 43kD(Calculated). |
| ユニプロット: | Q9NW38 |
| RRID: | AB_2842672 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9476, RRID:AB_2842672.
折りたたみ／展開
E3 ubiquitin-protein ligase FANCL; FAAP43; Fancl; FANCL_HUMAN; Fanconi anemia complementation group L; Fanconi anemia group L protein; Fanconi anemia-associated polypeptide of 43 kDa; FLJ10335; PHD finger protein 9; PHF9; POG; Ubiquitin ligase protein FANCL;
免疫原
- Q9NW38 FANCL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVTEASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFMMELKMLLEVALKNRQELYALPPPPQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NW38 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K139 | Sumoylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot |
研究背景
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
Cytoplasm. Nucleus.
Interacts with GGN (By similarity). Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. Interacts with FANCI. Directly interacts (via the RING-type zinc finger) with UBE2T and UBE2W.
The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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