LRSAM1 Antibody - #DF9480
| 製品: | LRSAM1 Antibody |
| カタログ: | DF9480 |
| タンパク質の説明: | Rabbit polyclonal antibody to LRSAM1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 84 kDa; 84kD(Calculated). |
| ユニプロット: | Q6UWE0 |
| RRID: | AB_2842676 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9480, RRID:AB_2842676.
折りたたみ／展開
CMT2P; E3 ubiquitin protein ligase LRSAM1; E3 ubiquitin-protein ligase LRSAM1; EC 6.3.2.-; FLJ31641; hTAL; Leucine rich repeat and sterile alpha motif containing 1; Leucine rich repeat and sterile alpha motif containing protein 1; Leucine-rich repeat and sterile alpha motif-containing protein 1; LRSAM1; LRSM1_HUMAN; OTTHUMP00000022174; OTTHUMP00000022175; RIFLE; TAL; Tsg101 associated ligase; Tsg101-associated ligase;
免疫原
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
- Q6UWE0 LRSM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEIPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELPDTVGELRSLRTLNISGNEIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCKNRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6UWE0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S604 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. Bacterial recognition protein that defends the cytoplasm from invasive pathogens. Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy).
Ubiquitination promoted by PHF23 leads to proteasomal degradation.
Cytoplasm.
Note: Displays a punctuate distribution and localizes to a submembranal ring (PubMed:15256501). Localizes to intracellular bacterial pathogens (PubMed:23245322).
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
Interacts with TSG101. Interacts with PHF23. Interacts with FUS.
The coiled coil domains interact with the SB domain of TSG101.
The PTAP motifs mediate the binding to UEV domains.
The LRR domain is necessary and sufficient for localization to bacterial targets.
The RING domain is required for ubiquitination.
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