RNF5 Antibody - #DF9490
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9490, RRID:AB_2842686.
折りたたみ／展開
E3 ubiquitin protein ligase RNF5; E3 ubiquitin-protein ligase RNF5; G16; HsRma1; NG2; OTTHUMP00000029107; Protein G16; Ram1 homolog; RING finger protein 5; RING5; RMA1; RNF 5; RNF5; RNF5_HUMAN;
免疫原
- Q99942 RNF5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99942 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot |
研究背景
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.
Membrane>Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane.
Note: Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
Widely expressed.
Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JKAMP. Interacts with STING1; the interaction of endogenous proteins is dependent on viral infection.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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